HSF-1-mediated cytoskeletal integrity determines thermotolerance and life span

Nathan A. Baird, Peter M. Douglas, Milos S. Simic, Ana R. Grant, James J. Moresco, Suzanne C. Wolff, John R. Yates, Gerard Manning, Andrew Dillin

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

The conserved heat shock transcription factor-1 (HSF-1) is essential to cellular stress resistance and life-span determination. The canonical function of HSF-1 is to regulate a network of genes encoding molecular chaperones that protect proteins from damage caused by extrinsic environmental stress or intrinsic age-related deterioration. In Caenorhabditis elegans, we engineered a modified HSF-1 strain that increased stress resistance and longevity without enhanced chaperone induction. This health assurance acted through the regulation of the calcium-binding protein PAT-10. Loss of pat-10 caused a collapse of the actin cytoskeleton, stress resistance, and life span. Furthermore, overexpression of pat-10 increased actin filament stability, thermotolerance, and longevity, indicating that in addition to chaperone regulation, HSF-1 has a prominent role in cytoskeletal integrity, ensuring cellular function during stress and aging.

Original languageEnglish (US)
Pages (from-to)360-363
Number of pages4
JournalScience
Volume346
Issue number6207
DOIs
StatePublished - Oct 17 2014

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Actin Cytoskeleton
Psychological Stress
Molecular Chaperones
Calcium-Binding Proteins
Gene Regulatory Networks
Caenorhabditis elegans
Thermotolerance
heat shock transcription factor
Health
Proteins

ASJC Scopus subject areas

  • General

Cite this

Baird, N. A., Douglas, P. M., Simic, M. S., Grant, A. R., Moresco, J. J., Wolff, S. C., ... Dillin, A. (2014). HSF-1-mediated cytoskeletal integrity determines thermotolerance and life span. Science, 346(6207), 360-363. https://doi.org/10.1126/science.1253168

HSF-1-mediated cytoskeletal integrity determines thermotolerance and life span. / Baird, Nathan A.; Douglas, Peter M.; Simic, Milos S.; Grant, Ana R.; Moresco, James J.; Wolff, Suzanne C.; Yates, John R.; Manning, Gerard; Dillin, Andrew.

In: Science, Vol. 346, No. 6207, 17.10.2014, p. 360-363.

Research output: Contribution to journalArticle

Baird, NA, Douglas, PM, Simic, MS, Grant, AR, Moresco, JJ, Wolff, SC, Yates, JR, Manning, G & Dillin, A 2014, 'HSF-1-mediated cytoskeletal integrity determines thermotolerance and life span', Science, vol. 346, no. 6207, pp. 360-363. https://doi.org/10.1126/science.1253168
Baird NA, Douglas PM, Simic MS, Grant AR, Moresco JJ, Wolff SC et al. HSF-1-mediated cytoskeletal integrity determines thermotolerance and life span. Science. 2014 Oct 17;346(6207):360-363. https://doi.org/10.1126/science.1253168
Baird, Nathan A. ; Douglas, Peter M. ; Simic, Milos S. ; Grant, Ana R. ; Moresco, James J. ; Wolff, Suzanne C. ; Yates, John R. ; Manning, Gerard ; Dillin, Andrew. / HSF-1-mediated cytoskeletal integrity determines thermotolerance and life span. In: Science. 2014 ; Vol. 346, No. 6207. pp. 360-363.
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