Human endomembrane H+ pump strongly resembles the ATP-synthetase of Archaebacteria

T. C. Sudhof, V. A. Fried, D. K. Stone, P. A. Johnston, X. S. Xie

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Abstract

Preparations of mammalian H+ pumps that acidify intracellular vesicles contain eight or nine polypeptides, ranging in size from 116 to 17 kDa. Biochemical analysis indicates that the 70- and 58-kDa polypeptides are subunits critical for ATP hydrolysis. The amino acid sequences of the major catalytic subunits (58 and 70 kDa) of the endomembrane H+ pump are unknown from animal cells. We report here the complete sequence of the 58-kDa subunit derived from a human kidney cDNA clone and partial sequences of the 70- and 58-kDa subunits purified from clathrin-coated vesicles of bovine brain. The amino acid sequences of both proteins strongly resemble the sequences of the corresponding subunits of the vacuolar H+ pumps of Archaebacteria, plants, and fungi. The archaebacterial enzyme is believed to use a H+ gradient to synthesize ATP. Thus, a common ancestral protein has given rise to a H+ pump that synthesizes ATP in one organism and hydrolyzes it in another and is highly conserved from prokaryotes to humans. The same pump appears to mediate the acidification of intracellular organelles, including coated vesicles, lysosomes, and secretory granules, as well as extracellular fluids such as urine.

Original languageEnglish (US)
Pages (from-to)6067-6071
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number16
StatePublished - 1989

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Proton Pumps
Archaea
Ligases
Adenosine Triphosphate
Amino Acid Sequence
Clathrin-Coated Vesicles
Coated Vesicles
Peptides
Extracellular Fluid
Secretory Vesicles
Lysosomes
Organelles
Catalytic Domain
Proteins
Hydrolysis
Fungi
Complementary DNA
Clone Cells
Urine
Kidney

ASJC Scopus subject areas

  • General
  • Genetics

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Human endomembrane H+ pump strongly resembles the ATP-synthetase of Archaebacteria. / Sudhof, T. C.; Fried, V. A.; Stone, D. K.; Johnston, P. A.; Xie, X. S.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, No. 16, 1989, p. 6067-6071.

Research output: Contribution to journalArticle

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AU - Xie, X. S.

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