Human polyomavirus 6 and 7 are associated with pruritic and dyskeratotic dermatoses

Khang D. Nguyen, Eunice E. Lee, Yangbo Yue, Jiri Stork, Lumir Pock, Jeffrey P. North, Travis Vandergriff, Clay Cockerell, Gregory A. Hosler, Diana V. Pastrana, Christopher B. Buck, Richard C. Wang

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Background: Human polyomavirus (HPyV)6 and HPyV7 are shed chronically from human skin. HPyV7, but not HPyV6, has been linked to a pruritic skin eruption of immunosuppression. Objective: We determined whether biopsy specimens showing a characteristic pattern of dyskeratosis and parakeratosis might be associated with polyomavirus infection. Methods: We screened biopsy specimens showing peacock plumage histology by polymerase chain reaction for HPyVs. Cases positive for HPyV6 or HPyV7 were then analyzed by immunohistochemistry, electron microscopy, immunofluorescence, quantitative polymerase chain reaction, and complete sequencing, including unbiased, next-generation sequencing. Results: We identified 3 additional cases of HPyV6 or HPyV7 skin infections. Expression of T antigen and viral capsid was abundant in lesional skin. Dual immunofluorescence staining experiments confirmed that HPyV7 primarily infects keratinocytes. High viral loads in lesional skin compared with normal-appearing skin and the identification of intact virions by both electron microscopy and next-generation sequencing support a role for active viral infections in these skin diseases. Limitation: This was a small case series of archived materials. Conclusion: We have found that HPyV6 and HPyV7 are associated with rare, pruritic skin eruptions with a distinctive histologic pattern and describe this entity as HPyV6- and HPyV7-associated pruritic and dyskeratotic dermatoses.

Original languageEnglish (US)
JournalJournal of the American Academy of Dermatology
DOIs
StateAccepted/In press - 2016

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Polyomavirus
Skin Diseases
Skin
Fluorescent Antibody Technique
Electron Microscopy
Polyomavirus Infections
Parakeratosis
Biopsy
Polymerase Chain Reaction
Capsid
Viral Tumor Antigens
Virus Diseases
Viral Load
Keratinocytes
Virion
Immunosuppression
Histology
Immunohistochemistry
Staining and Labeling
Infection

Keywords

  • Dyskeratosis
  • HIV/AIDS
  • Human polyomavirus 6
  • Human polyomavirus 7
  • Immunosuppression
  • Organ transplantation
  • Parakeratosis
  • Polyomavirus
  • Pruritus

ASJC Scopus subject areas

  • Dermatology

Cite this

Human polyomavirus 6 and 7 are associated with pruritic and dyskeratotic dermatoses. / Nguyen, Khang D.; Lee, Eunice E.; Yue, Yangbo; Stork, Jiri; Pock, Lumir; North, Jeffrey P.; Vandergriff, Travis; Cockerell, Clay; Hosler, Gregory A.; Pastrana, Diana V.; Buck, Christopher B.; Wang, Richard C.

In: Journal of the American Academy of Dermatology, 2016.

Research output: Contribution to journalArticle

Nguyen, Khang D. ; Lee, Eunice E. ; Yue, Yangbo ; Stork, Jiri ; Pock, Lumir ; North, Jeffrey P. ; Vandergriff, Travis ; Cockerell, Clay ; Hosler, Gregory A. ; Pastrana, Diana V. ; Buck, Christopher B. ; Wang, Richard C. / Human polyomavirus 6 and 7 are associated with pruritic and dyskeratotic dermatoses. In: Journal of the American Academy of Dermatology. 2016.
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abstract = "Background: Human polyomavirus (HPyV)6 and HPyV7 are shed chronically from human skin. HPyV7, but not HPyV6, has been linked to a pruritic skin eruption of immunosuppression. Objective: We determined whether biopsy specimens showing a characteristic pattern of dyskeratosis and parakeratosis might be associated with polyomavirus infection. Methods: We screened biopsy specimens showing peacock plumage histology by polymerase chain reaction for HPyVs. Cases positive for HPyV6 or HPyV7 were then analyzed by immunohistochemistry, electron microscopy, immunofluorescence, quantitative polymerase chain reaction, and complete sequencing, including unbiased, next-generation sequencing. Results: We identified 3 additional cases of HPyV6 or HPyV7 skin infections. Expression of T antigen and viral capsid was abundant in lesional skin. Dual immunofluorescence staining experiments confirmed that HPyV7 primarily infects keratinocytes. High viral loads in lesional skin compared with normal-appearing skin and the identification of intact virions by both electron microscopy and next-generation sequencing support a role for active viral infections in these skin diseases. Limitation: This was a small case series of archived materials. Conclusion: We have found that HPyV6 and HPyV7 are associated with rare, pruritic skin eruptions with a distinctive histologic pattern and describe this entity as HPyV6- and HPyV7-associated pruritic and dyskeratotic dermatoses.",
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AU - Nguyen, Khang D.

AU - Lee, Eunice E.

AU - Yue, Yangbo

AU - Stork, Jiri

AU - Pock, Lumir

AU - North, Jeffrey P.

AU - Vandergriff, Travis

AU - Cockerell, Clay

AU - Hosler, Gregory A.

AU - Pastrana, Diana V.

AU - Buck, Christopher B.

AU - Wang, Richard C.

PY - 2016

Y1 - 2016

N2 - Background: Human polyomavirus (HPyV)6 and HPyV7 are shed chronically from human skin. HPyV7, but not HPyV6, has been linked to a pruritic skin eruption of immunosuppression. Objective: We determined whether biopsy specimens showing a characteristic pattern of dyskeratosis and parakeratosis might be associated with polyomavirus infection. Methods: We screened biopsy specimens showing peacock plumage histology by polymerase chain reaction for HPyVs. Cases positive for HPyV6 or HPyV7 were then analyzed by immunohistochemistry, electron microscopy, immunofluorescence, quantitative polymerase chain reaction, and complete sequencing, including unbiased, next-generation sequencing. Results: We identified 3 additional cases of HPyV6 or HPyV7 skin infections. Expression of T antigen and viral capsid was abundant in lesional skin. Dual immunofluorescence staining experiments confirmed that HPyV7 primarily infects keratinocytes. High viral loads in lesional skin compared with normal-appearing skin and the identification of intact virions by both electron microscopy and next-generation sequencing support a role for active viral infections in these skin diseases. Limitation: This was a small case series of archived materials. Conclusion: We have found that HPyV6 and HPyV7 are associated with rare, pruritic skin eruptions with a distinctive histologic pattern and describe this entity as HPyV6- and HPyV7-associated pruritic and dyskeratotic dermatoses.

AB - Background: Human polyomavirus (HPyV)6 and HPyV7 are shed chronically from human skin. HPyV7, but not HPyV6, has been linked to a pruritic skin eruption of immunosuppression. Objective: We determined whether biopsy specimens showing a characteristic pattern of dyskeratosis and parakeratosis might be associated with polyomavirus infection. Methods: We screened biopsy specimens showing peacock plumage histology by polymerase chain reaction for HPyVs. Cases positive for HPyV6 or HPyV7 were then analyzed by immunohistochemistry, electron microscopy, immunofluorescence, quantitative polymerase chain reaction, and complete sequencing, including unbiased, next-generation sequencing. Results: We identified 3 additional cases of HPyV6 or HPyV7 skin infections. Expression of T antigen and viral capsid was abundant in lesional skin. Dual immunofluorescence staining experiments confirmed that HPyV7 primarily infects keratinocytes. High viral loads in lesional skin compared with normal-appearing skin and the identification of intact virions by both electron microscopy and next-generation sequencing support a role for active viral infections in these skin diseases. Limitation: This was a small case series of archived materials. Conclusion: We have found that HPyV6 and HPyV7 are associated with rare, pruritic skin eruptions with a distinctive histologic pattern and describe this entity as HPyV6- and HPyV7-associated pruritic and dyskeratotic dermatoses.

KW - Dyskeratosis

KW - HIV/AIDS

KW - Human polyomavirus 6

KW - Human polyomavirus 7

KW - Immunosuppression

KW - Organ transplantation

KW - Parakeratosis

KW - Polyomavirus

KW - Pruritus

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