HuR expression in the nucleus correlates with high histological grade and poor disease-free survival in ovarian cancer

Xiaofang Yi, Yi Zhou, Wenxin Zheng, Setsuko K. Chambers

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Background: HuR, a nucleo-cytoplasmic shuttling protein, plays an important role in mRNA stability as well as cellular differentiation. Recently, HuR expression, particularly in the cytoplasm, was thought to be associated with the prognosis of several cancers including ovarian cancer. Aims: To study the clinical significance of nuclear HuR expression in ovarian cancer. Methods: Primary epithelial ovarian carcinomas (102) and ovarian low malignant potential tumours (11) were assessed for HuR protein expression by immunohistochemistry. HuR scoring accounted for both intensity and percentage of cells stained, and ranged from 0 to 300. Results: HuR was found to be present predominantly in the nucleus, where it was expressed in 85.8% of cases. Nuclear HuR was associated with the invasive cancers (P = 0.004), high grade (P < 0.0001), large residual disease (P = 0.045) and poor disease-free survival (P = 0.0009). Among those 91 specimens with high grade, 76.9% had a high nuclear HuR score, while in those 22 cases with low grade, only 31.8% had a high HuR score (P < 0.0001). Multivariate analysis showed that nuclear HuR intensity was an independent prognostic factor for poor disease-free survival (P = 0.0484). When the invasive cancers were analysed separately, only the association between nuclear HuR and high grade remained (P = 0.0089). Conclusions: Our results support the clinical significance of nuclear HuR in ovarian carcinoma and suggest that nuclear HuR may also play a role in the biology of ovarian cancer. These data suggest a more complex model for HuR in ovarian cancer than one limited to cytoplasmic localisation.

Original languageEnglish (US)
Pages (from-to)93-98
Number of pages6
JournalAustralian and New Zealand Journal of Obstetrics and Gynaecology
Volume49
Issue number1
DOIs
StatePublished - Feb 1 2009

Fingerprint

Ovarian Neoplasms
Disease-Free Survival
Neoplasms
Carcinoma
RNA Stability
Cytoplasm
Multivariate Analysis
Immunohistochemistry
Proteins

Keywords

  • Histological grade
  • HuR antigen
  • Ovarian neoplasms

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

HuR expression in the nucleus correlates with high histological grade and poor disease-free survival in ovarian cancer. / Yi, Xiaofang; Zhou, Yi; Zheng, Wenxin; Chambers, Setsuko K.

In: Australian and New Zealand Journal of Obstetrics and Gynaecology, Vol. 49, No. 1, 01.02.2009, p. 93-98.

Research output: Contribution to journalArticle

@article{a16ded2c15d7451287f10343426d84ea,
title = "HuR expression in the nucleus correlates with high histological grade and poor disease-free survival in ovarian cancer",
abstract = "Background: HuR, a nucleo-cytoplasmic shuttling protein, plays an important role in mRNA stability as well as cellular differentiation. Recently, HuR expression, particularly in the cytoplasm, was thought to be associated with the prognosis of several cancers including ovarian cancer. Aims: To study the clinical significance of nuclear HuR expression in ovarian cancer. Methods: Primary epithelial ovarian carcinomas (102) and ovarian low malignant potential tumours (11) were assessed for HuR protein expression by immunohistochemistry. HuR scoring accounted for both intensity and percentage of cells stained, and ranged from 0 to 300. Results: HuR was found to be present predominantly in the nucleus, where it was expressed in 85.8{\%} of cases. Nuclear HuR was associated with the invasive cancers (P = 0.004), high grade (P < 0.0001), large residual disease (P = 0.045) and poor disease-free survival (P = 0.0009). Among those 91 specimens with high grade, 76.9{\%} had a high nuclear HuR score, while in those 22 cases with low grade, only 31.8{\%} had a high HuR score (P < 0.0001). Multivariate analysis showed that nuclear HuR intensity was an independent prognostic factor for poor disease-free survival (P = 0.0484). When the invasive cancers were analysed separately, only the association between nuclear HuR and high grade remained (P = 0.0089). Conclusions: Our results support the clinical significance of nuclear HuR in ovarian carcinoma and suggest that nuclear HuR may also play a role in the biology of ovarian cancer. These data suggest a more complex model for HuR in ovarian cancer than one limited to cytoplasmic localisation.",
keywords = "Histological grade, HuR antigen, Ovarian neoplasms",
author = "Xiaofang Yi and Yi Zhou and Wenxin Zheng and Chambers, {Setsuko K.}",
year = "2009",
month = "2",
day = "1",
doi = "10.1111/j.1479-828X.2008.00937.x",
language = "English (US)",
volume = "49",
pages = "93--98",
journal = "Australian and New Zealand Journal of Obstetrics and Gynaecology",
issn = "0004-8666",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - HuR expression in the nucleus correlates with high histological grade and poor disease-free survival in ovarian cancer

AU - Yi, Xiaofang

AU - Zhou, Yi

AU - Zheng, Wenxin

AU - Chambers, Setsuko K.

PY - 2009/2/1

Y1 - 2009/2/1

N2 - Background: HuR, a nucleo-cytoplasmic shuttling protein, plays an important role in mRNA stability as well as cellular differentiation. Recently, HuR expression, particularly in the cytoplasm, was thought to be associated with the prognosis of several cancers including ovarian cancer. Aims: To study the clinical significance of nuclear HuR expression in ovarian cancer. Methods: Primary epithelial ovarian carcinomas (102) and ovarian low malignant potential tumours (11) were assessed for HuR protein expression by immunohistochemistry. HuR scoring accounted for both intensity and percentage of cells stained, and ranged from 0 to 300. Results: HuR was found to be present predominantly in the nucleus, where it was expressed in 85.8% of cases. Nuclear HuR was associated with the invasive cancers (P = 0.004), high grade (P < 0.0001), large residual disease (P = 0.045) and poor disease-free survival (P = 0.0009). Among those 91 specimens with high grade, 76.9% had a high nuclear HuR score, while in those 22 cases with low grade, only 31.8% had a high HuR score (P < 0.0001). Multivariate analysis showed that nuclear HuR intensity was an independent prognostic factor for poor disease-free survival (P = 0.0484). When the invasive cancers were analysed separately, only the association between nuclear HuR and high grade remained (P = 0.0089). Conclusions: Our results support the clinical significance of nuclear HuR in ovarian carcinoma and suggest that nuclear HuR may also play a role in the biology of ovarian cancer. These data suggest a more complex model for HuR in ovarian cancer than one limited to cytoplasmic localisation.

AB - Background: HuR, a nucleo-cytoplasmic shuttling protein, plays an important role in mRNA stability as well as cellular differentiation. Recently, HuR expression, particularly in the cytoplasm, was thought to be associated with the prognosis of several cancers including ovarian cancer. Aims: To study the clinical significance of nuclear HuR expression in ovarian cancer. Methods: Primary epithelial ovarian carcinomas (102) and ovarian low malignant potential tumours (11) were assessed for HuR protein expression by immunohistochemistry. HuR scoring accounted for both intensity and percentage of cells stained, and ranged from 0 to 300. Results: HuR was found to be present predominantly in the nucleus, where it was expressed in 85.8% of cases. Nuclear HuR was associated with the invasive cancers (P = 0.004), high grade (P < 0.0001), large residual disease (P = 0.045) and poor disease-free survival (P = 0.0009). Among those 91 specimens with high grade, 76.9% had a high nuclear HuR score, while in those 22 cases with low grade, only 31.8% had a high HuR score (P < 0.0001). Multivariate analysis showed that nuclear HuR intensity was an independent prognostic factor for poor disease-free survival (P = 0.0484). When the invasive cancers were analysed separately, only the association between nuclear HuR and high grade remained (P = 0.0089). Conclusions: Our results support the clinical significance of nuclear HuR in ovarian carcinoma and suggest that nuclear HuR may also play a role in the biology of ovarian cancer. These data suggest a more complex model for HuR in ovarian cancer than one limited to cytoplasmic localisation.

KW - Histological grade

KW - HuR antigen

KW - Ovarian neoplasms

UR - http://www.scopus.com/inward/record.url?scp=66549086896&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=66549086896&partnerID=8YFLogxK

U2 - 10.1111/j.1479-828X.2008.00937.x

DO - 10.1111/j.1479-828X.2008.00937.x

M3 - Article

C2 - 19281587

AN - SCOPUS:66549086896

VL - 49

SP - 93

EP - 98

JO - Australian and New Zealand Journal of Obstetrics and Gynaecology

JF - Australian and New Zealand Journal of Obstetrics and Gynaecology

SN - 0004-8666

IS - 1

ER -