HuR mRNA ligands expressed after seizure

Cara J. Westmark, Françoise A. Gourronc, Virginia B. Bartleson, Ümit Sayin, Saswati Bhattacharya, Tom Sutula, James S. Malter

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

HuR is a ubiquitously expressed AU-rich element (ARE)-binding protein that interacts with and stabilizes selective early response gene (ERG) mRNAs after cell activation or stress. To date, approximately 20 mRNAs have been unambiguously defined as HuR ligands. Given the discordance between the large number of ERG mRNAs and those few defined as ligands, we applied in vitro selection to isolate a broad range of HuR mRNA ligands using postseizure mouse hippocampal tissue. Selected mRNAs were converted into cDNA libraries and sequenced. Using this approach, we have identified over 600 novel, putative HuR mRNA ligands. These genes code for a variety of proteins, including transcription factors, signaling molecules, and kinases, but many have unknown function. Consistent with the means of their selection, several of these HuR ligands are differentially expressed in hippocampus after seizure. These results demonstrate a biochemical approach to identify and characterize the diverse repertoire of ligands for HuR and other regulatory mRNA-binding proteins.

Original languageEnglish (US)
Pages (from-to)1037-1045
Number of pages9
JournalJournal of neuropathology and experimental neurology
Volume64
Issue number12
DOIs
StatePublished - Dec 1 2005

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Keywords

  • Early response gene
  • Epilepsy
  • HuR
  • Pentylenetetrazole

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Neurology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

Cite this

Westmark, C. J., Gourronc, F. A., Bartleson, V. B., Sayin, Ü., Bhattacharya, S., Sutula, T., & Malter, J. S. (2005). HuR mRNA ligands expressed after seizure. Journal of neuropathology and experimental neurology, 64(12), 1037-1045. https://doi.org/10.1097/01.jnen.0000189835.71574.e1