TY - JOUR
T1 - Hyperpolarized [1,4-13C]-diethylsuccinate
T2 - A potential DNP substrate for in vivo metabolic imaging
AU - Billingsley, Kelvin L.
AU - Josan, Sonal
AU - Park, Jae Mo
AU - Tee, Sui Seng
AU - Spielman-Sun, Eleanor
AU - Hurd, Ralph
AU - Mayer, Dirk
AU - Spielman, Daniel
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2014/3
Y1 - 2014/3
N2 - The tricarboxylic acid (TCA) cycle performs an essential role in the regulation of energy and metabolism, and deficiencies in this pathway are commonly correlated with various diseases. However, the development of non-invasive techniques for the assessment of the cycle in vivo has remained challenging. In this work, the applicability of a novel imaging agent, [1,4-13C]-diethylsuccinate, for hyperpolarized 13C metabolic imaging of the TCA cycle was explored. In vivo spectroscopic studies were conducted in conjunction with in vitro analyses to determine the metabolic fate of the imaging agent. Contrary to previous reports (Zacharias NM et al. J. Am. Chem. Soc. 2012; 134: 934-943), [13C]-labeled diethylsuccinate was primarily metabolized to succinate-derived products not originating from TCA cycle metabolism. These results illustrate potential issues of utilizing dialkyl ester analogs of TCA cycle intermediates as molecular probes for hyperpolarized 13C metabolic imaging.
AB - The tricarboxylic acid (TCA) cycle performs an essential role in the regulation of energy and metabolism, and deficiencies in this pathway are commonly correlated with various diseases. However, the development of non-invasive techniques for the assessment of the cycle in vivo has remained challenging. In this work, the applicability of a novel imaging agent, [1,4-13C]-diethylsuccinate, for hyperpolarized 13C metabolic imaging of the TCA cycle was explored. In vivo spectroscopic studies were conducted in conjunction with in vitro analyses to determine the metabolic fate of the imaging agent. Contrary to previous reports (Zacharias NM et al. J. Am. Chem. Soc. 2012; 134: 934-943), [13C]-labeled diethylsuccinate was primarily metabolized to succinate-derived products not originating from TCA cycle metabolism. These results illustrate potential issues of utilizing dialkyl ester analogs of TCA cycle intermediates as molecular probes for hyperpolarized 13C metabolic imaging.
KW - Dynamic nuclear polarization
KW - Hyperpolarized carbon-13
KW - Magnetic resonance spectroscopy
KW - Tricarboxylic acid cycle
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U2 - 10.1002/nbm.3071
DO - 10.1002/nbm.3071
M3 - Article
C2 - 24421249
AN - SCOPUS:84893723311
VL - 27
SP - 356
EP - 362
JO - NMR in Biomedicine
JF - NMR in Biomedicine
SN - 0952-3480
IS - 3
ER -