Identification of a heritable model of testicular germ cell tumor in the zebrafish

Joanie C. Neumann, Jennifer Shepard Dovey, Garvin L. Chandler, Liliana Carbajal, James F. Amatruda

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Germ cell tumors (GCTs) affect infants, children, and adults and are the most common cancer type in young men. Progress in understanding the molecular basis of GCTs has been hampered by a lack of suitable animal models. Here we report the identification of a zebrafish model of highly penetrant, heritable testicular GCT isolated as part of a forward genetic screen for cancer susceptibility genes. The mutant line develops spontaneous testicular tumors at a median age of 7 months, and pedigree analysis indicates dominant inheritance of the GCT susceptibility trait. The zebrafish model exhibits disruption of testicular tissue architecture and the accumulation of primitive, spermatogonial-like cells with loss of spermatocytic differentiation. Radiation treatment leads to apoptosis of the tumor cells and tumor regression. The GCT-susceptible line can serve as a model for understanding the mechanisms regulating germ cells in normal development and disease and as a platform investigating new therapeutic approaches for GCTs.

Original languageEnglish (US)
Pages (from-to)319-327
Number of pages9
JournalZebrafish
Volume6
Issue number4
DOIs
StatePublished - Dec 1 2009

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Developmental Biology

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    Neumann, J. C., Dovey, J. S., Chandler, G. L., Carbajal, L., & Amatruda, J. F. (2009). Identification of a heritable model of testicular germ cell tumor in the zebrafish. Zebrafish, 6(4), 319-327. https://doi.org/10.1089/zeb.2009.0613