Identification of a heritable model of testicular germ cell tumor in the zebrafish

Joanie C. Neumann, Jennifer Shepard Dovey, Garvin L. Chandler, Liliana Carbajal, James F. Amatruda

Research output: Contribution to journalArticle

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Abstract

Germ cell tumors (GCTs) affect infants, children, and adults and are the most common cancer type in young men. Progress in understanding the molecular basis of GCTs has been hampered by a lack of suitable animal models. Here we report the identification of a zebrafish model of highly penetrant, heritable testicular GCT isolated as part of a forward genetic screen for cancer susceptibility genes. The mutant line develops spontaneous testicular tumors at a median age of 7 months, and pedigree analysis indicates dominant inheritance of the GCT susceptibility trait. The zebrafish model exhibits disruption of testicular tissue architecture and the accumulation of primitive, spermatogonial-like cells with loss of spermatocytic differentiation. Radiation treatment leads to apoptosis of the tumor cells and tumor regression. The GCT-susceptible line can serve as a model for understanding the mechanisms regulating germ cells in normal development and disease and as a platform investigating new therapeutic approaches for GCTs.

Original languageEnglish (US)
Pages (from-to)319-327
Number of pages9
JournalZebrafish
Volume6
Issue number4
DOIs
StatePublished - Dec 1 2009

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Germ Cell and Embryonal Neoplasms
Zebrafish
Danio rerio
germ cells
neoplasms
Neoplasms
Neoplasm Genes
Testicular Neoplasms
Pedigree
Germ Cells
Animal Models
Testicular Germ Cell Tumor
Radiation
Apoptosis
remission
pedigree
Therapeutics
inheritance (genetics)
apoptosis
animal models

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Developmental Biology

Cite this

Neumann, J. C., Dovey, J. S., Chandler, G. L., Carbajal, L., & Amatruda, J. F. (2009). Identification of a heritable model of testicular germ cell tumor in the zebrafish. Zebrafish, 6(4), 319-327. https://doi.org/10.1089/zeb.2009.0613

Identification of a heritable model of testicular germ cell tumor in the zebrafish. / Neumann, Joanie C.; Dovey, Jennifer Shepard; Chandler, Garvin L.; Carbajal, Liliana; Amatruda, James F.

In: Zebrafish, Vol. 6, No. 4, 01.12.2009, p. 319-327.

Research output: Contribution to journalArticle

Neumann, JC, Dovey, JS, Chandler, GL, Carbajal, L & Amatruda, JF 2009, 'Identification of a heritable model of testicular germ cell tumor in the zebrafish', Zebrafish, vol. 6, no. 4, pp. 319-327. https://doi.org/10.1089/zeb.2009.0613
Neumann JC, Dovey JS, Chandler GL, Carbajal L, Amatruda JF. Identification of a heritable model of testicular germ cell tumor in the zebrafish. Zebrafish. 2009 Dec 1;6(4):319-327. https://doi.org/10.1089/zeb.2009.0613
Neumann, Joanie C. ; Dovey, Jennifer Shepard ; Chandler, Garvin L. ; Carbajal, Liliana ; Amatruda, James F. / Identification of a heritable model of testicular germ cell tumor in the zebrafish. In: Zebrafish. 2009 ; Vol. 6, No. 4. pp. 319-327.
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