Identification of a novel tumor suppressor gene p34 on human chromosome 6q25.1

Min Wang, Haris G. Vikis, Yian Wang, Dongmei Jia, Daolong Wang, Laura J. Bierut, Joan E. Bailey-Wilson, Christopher I. Amos, Susan M. Pinney, Gloria M. Petersen, Mariza De Andrade, Ping Yang, Jonathan S. Wiest, Pamela R. Fain, Ann G. Schwartz, Adi Gazdar, John Minna, Colette Gaba, Henry Rothschild, Diptasri Mandal & 7 others Elena Kupert, Daniela Seminara, Yan Liu, Avinash Viswanathan, Ramaswamy Govindan, Marshall W. Anderson, Ming You

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

In this study, we observed loss of heterozygosity (LOH) in human chromosomal fragment 6q25.1 in sporadic lung cancer patients. LOH was observed in 65% of the 26 lung tumors examined and was narrowed down to a 2.2-Mb region. Single-nucleotide polymorphism (SNP) analysis of genes located within this region identified a candidate gene, termed p34. This gene, also designated as ZC3H12D, C6orf95, FLJ46041, or dJ281H8.1, carries an A/G nonsynonymous SNP at codon 106, which alters the amino acid from lysine to argmine. Nearly 73% of heterozygous lung cancer tissues with LOH and the A/G SNP also exhibited loss of the A allele. In vitro clonogenic and in vivo nude mouse studies showed that overexpression of the A allele exerts tumor suppressor function compared with the G allele. p34 is located within a recently mapped human lung cancer susceptibility locus, and association of the p34 A/G SNP was tested among these families. No significant association between the less frequent G allele and lung cancer susceptibility was found. Our results suggest that p34 may be a novel tumor suppressor gene involved in sporadic lung cancer but it seems not to be the candidate familial lung cancer susceptibility gene linked to chromosomal region 6q23-25.

Original languageEnglish (US)
Pages (from-to)93-99
Number of pages7
JournalCancer Research
Volume67
Issue number1
DOIs
StatePublished - Jan 1 2007

Fingerprint

Human Chromosomes
Tumor Suppressor Genes
Lung Neoplasms
Single Nucleotide Polymorphism
Loss of Heterozygosity
Alleles
Genes
Neoplasm Genes
Nude Mice
Codon
Lysine
Neoplasms
Amino Acids
Lung

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Wang, M., Vikis, H. G., Wang, Y., Jia, D., Wang, D., Bierut, L. J., ... You, M. (2007). Identification of a novel tumor suppressor gene p34 on human chromosome 6q25.1. Cancer Research, 67(1), 93-99. https://doi.org/10.1158/0008-5472.CAN-06-2723

Identification of a novel tumor suppressor gene p34 on human chromosome 6q25.1. / Wang, Min; Vikis, Haris G.; Wang, Yian; Jia, Dongmei; Wang, Daolong; Bierut, Laura J.; Bailey-Wilson, Joan E.; Amos, Christopher I.; Pinney, Susan M.; Petersen, Gloria M.; De Andrade, Mariza; Yang, Ping; Wiest, Jonathan S.; Fain, Pamela R.; Schwartz, Ann G.; Gazdar, Adi; Minna, John; Gaba, Colette; Rothschild, Henry; Mandal, Diptasri; Kupert, Elena; Seminara, Daniela; Liu, Yan; Viswanathan, Avinash; Govindan, Ramaswamy; Anderson, Marshall W.; You, Ming.

In: Cancer Research, Vol. 67, No. 1, 01.01.2007, p. 93-99.

Research output: Contribution to journalArticle

Wang, M, Vikis, HG, Wang, Y, Jia, D, Wang, D, Bierut, LJ, Bailey-Wilson, JE, Amos, CI, Pinney, SM, Petersen, GM, De Andrade, M, Yang, P, Wiest, JS, Fain, PR, Schwartz, AG, Gazdar, A, Minna, J, Gaba, C, Rothschild, H, Mandal, D, Kupert, E, Seminara, D, Liu, Y, Viswanathan, A, Govindan, R, Anderson, MW & You, M 2007, 'Identification of a novel tumor suppressor gene p34 on human chromosome 6q25.1', Cancer Research, vol. 67, no. 1, pp. 93-99. https://doi.org/10.1158/0008-5472.CAN-06-2723
Wang, Min ; Vikis, Haris G. ; Wang, Yian ; Jia, Dongmei ; Wang, Daolong ; Bierut, Laura J. ; Bailey-Wilson, Joan E. ; Amos, Christopher I. ; Pinney, Susan M. ; Petersen, Gloria M. ; De Andrade, Mariza ; Yang, Ping ; Wiest, Jonathan S. ; Fain, Pamela R. ; Schwartz, Ann G. ; Gazdar, Adi ; Minna, John ; Gaba, Colette ; Rothschild, Henry ; Mandal, Diptasri ; Kupert, Elena ; Seminara, Daniela ; Liu, Yan ; Viswanathan, Avinash ; Govindan, Ramaswamy ; Anderson, Marshall W. ; You, Ming. / Identification of a novel tumor suppressor gene p34 on human chromosome 6q25.1. In: Cancer Research. 2007 ; Vol. 67, No. 1. pp. 93-99.
@article{ae4052a875f846d5b1d8a9c328481a29,
title = "Identification of a novel tumor suppressor gene p34 on human chromosome 6q25.1",
abstract = "In this study, we observed loss of heterozygosity (LOH) in human chromosomal fragment 6q25.1 in sporadic lung cancer patients. LOH was observed in 65{\%} of the 26 lung tumors examined and was narrowed down to a 2.2-Mb region. Single-nucleotide polymorphism (SNP) analysis of genes located within this region identified a candidate gene, termed p34. This gene, also designated as ZC3H12D, C6orf95, FLJ46041, or dJ281H8.1, carries an A/G nonsynonymous SNP at codon 106, which alters the amino acid from lysine to argmine. Nearly 73{\%} of heterozygous lung cancer tissues with LOH and the A/G SNP also exhibited loss of the A allele. In vitro clonogenic and in vivo nude mouse studies showed that overexpression of the A allele exerts tumor suppressor function compared with the G allele. p34 is located within a recently mapped human lung cancer susceptibility locus, and association of the p34 A/G SNP was tested among these families. No significant association between the less frequent G allele and lung cancer susceptibility was found. Our results suggest that p34 may be a novel tumor suppressor gene involved in sporadic lung cancer but it seems not to be the candidate familial lung cancer susceptibility gene linked to chromosomal region 6q23-25.",
author = "Min Wang and Vikis, {Haris G.} and Yian Wang and Dongmei Jia and Daolong Wang and Bierut, {Laura J.} and Bailey-Wilson, {Joan E.} and Amos, {Christopher I.} and Pinney, {Susan M.} and Petersen, {Gloria M.} and {De Andrade}, Mariza and Ping Yang and Wiest, {Jonathan S.} and Fain, {Pamela R.} and Schwartz, {Ann G.} and Adi Gazdar and John Minna and Colette Gaba and Henry Rothschild and Diptasri Mandal and Elena Kupert and Daniela Seminara and Yan Liu and Avinash Viswanathan and Ramaswamy Govindan and Anderson, {Marshall W.} and Ming You",
year = "2007",
month = "1",
day = "1",
doi = "10.1158/0008-5472.CAN-06-2723",
language = "English (US)",
volume = "67",
pages = "93--99",
journal = "Journal of Cancer Research",
issn = "0099-7013",
publisher = "American Association for Cancer Research Inc.",
number = "1",

}

TY - JOUR

T1 - Identification of a novel tumor suppressor gene p34 on human chromosome 6q25.1

AU - Wang, Min

AU - Vikis, Haris G.

AU - Wang, Yian

AU - Jia, Dongmei

AU - Wang, Daolong

AU - Bierut, Laura J.

AU - Bailey-Wilson, Joan E.

AU - Amos, Christopher I.

AU - Pinney, Susan M.

AU - Petersen, Gloria M.

AU - De Andrade, Mariza

AU - Yang, Ping

AU - Wiest, Jonathan S.

AU - Fain, Pamela R.

AU - Schwartz, Ann G.

AU - Gazdar, Adi

AU - Minna, John

AU - Gaba, Colette

AU - Rothschild, Henry

AU - Mandal, Diptasri

AU - Kupert, Elena

AU - Seminara, Daniela

AU - Liu, Yan

AU - Viswanathan, Avinash

AU - Govindan, Ramaswamy

AU - Anderson, Marshall W.

AU - You, Ming

PY - 2007/1/1

Y1 - 2007/1/1

N2 - In this study, we observed loss of heterozygosity (LOH) in human chromosomal fragment 6q25.1 in sporadic lung cancer patients. LOH was observed in 65% of the 26 lung tumors examined and was narrowed down to a 2.2-Mb region. Single-nucleotide polymorphism (SNP) analysis of genes located within this region identified a candidate gene, termed p34. This gene, also designated as ZC3H12D, C6orf95, FLJ46041, or dJ281H8.1, carries an A/G nonsynonymous SNP at codon 106, which alters the amino acid from lysine to argmine. Nearly 73% of heterozygous lung cancer tissues with LOH and the A/G SNP also exhibited loss of the A allele. In vitro clonogenic and in vivo nude mouse studies showed that overexpression of the A allele exerts tumor suppressor function compared with the G allele. p34 is located within a recently mapped human lung cancer susceptibility locus, and association of the p34 A/G SNP was tested among these families. No significant association between the less frequent G allele and lung cancer susceptibility was found. Our results suggest that p34 may be a novel tumor suppressor gene involved in sporadic lung cancer but it seems not to be the candidate familial lung cancer susceptibility gene linked to chromosomal region 6q23-25.

AB - In this study, we observed loss of heterozygosity (LOH) in human chromosomal fragment 6q25.1 in sporadic lung cancer patients. LOH was observed in 65% of the 26 lung tumors examined and was narrowed down to a 2.2-Mb region. Single-nucleotide polymorphism (SNP) analysis of genes located within this region identified a candidate gene, termed p34. This gene, also designated as ZC3H12D, C6orf95, FLJ46041, or dJ281H8.1, carries an A/G nonsynonymous SNP at codon 106, which alters the amino acid from lysine to argmine. Nearly 73% of heterozygous lung cancer tissues with LOH and the A/G SNP also exhibited loss of the A allele. In vitro clonogenic and in vivo nude mouse studies showed that overexpression of the A allele exerts tumor suppressor function compared with the G allele. p34 is located within a recently mapped human lung cancer susceptibility locus, and association of the p34 A/G SNP was tested among these families. No significant association between the less frequent G allele and lung cancer susceptibility was found. Our results suggest that p34 may be a novel tumor suppressor gene involved in sporadic lung cancer but it seems not to be the candidate familial lung cancer susceptibility gene linked to chromosomal region 6q23-25.

UR - http://www.scopus.com/inward/record.url?scp=33846408685&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846408685&partnerID=8YFLogxK

U2 - 10.1158/0008-5472.CAN-06-2723

DO - 10.1158/0008-5472.CAN-06-2723

M3 - Article

VL - 67

SP - 93

EP - 99

JO - Journal of Cancer Research

JF - Journal of Cancer Research

SN - 0099-7013

IS - 1

ER -