Identification of candidate tumor suppressor genes in childhood acute lymphoblastic leukemia at chromosome 6q16.3-21 in China

Rui Kang, Li Zhi Cao, Yan Yu, Ming Hua Yang, Zhao Xia Zhang, Bi Yun Guo, Min Xie, Ying Chen, Zhi Hong Tan, Zhuo Wang, Ting Hu, Xiu Shan Wu

Research output: Contribution to journalArticle

Abstract

In order to identify candidate tumor suppressor genes (TSGs) in childhood acute lymphoblastic leukemia (ALL), firstly, loss of heterozygosity (LOH) of 6q16.3-21 in 139 primary ALL samples was analyzed by using polymerase chain reaction (PCR) and 11 microsatellite markers. The frequency of LOH on 6q16.3-21 was 32%. A 2-cM high frequency deletion region was flanked by D6S1709 and D6S301 loci at 6q16.3-21. Clinical data showed that patient with 6q16.3-21 LOH had higher WBC counts and blast cells (P < 0.05). The statistics about age, sex, classification of morphology and immunology were indistinct (P > 0.05). Then, positional cloning strategy, bioinformatics technology and reverse transcription-polymerase chain reaction (RT-PCR) were used to identify candidate TSGs and its cDNA fragments at 6q16.3-21, especially at the high frequency deletion region. Comparing with expression of normal peripheral blood mononuclear cell, EST screened in D6S1709-D6S301 (GenBank Accession No.AA403058) was down-regulation in ten of fifteen childhood ALL (P < 0.05). Digital differential display showed that the expression levels of AMD1, PPIL6 and WASF1 were lower in cancer tissues than in normal tissues (P < 0.05). These findings may provide new clues in cloning of childhood ALL TSGs at 6q16.3-21.

Original languageEnglish (US)
Pages (from-to)65-71
Number of pages7
JournalProgress in Biochemistry and Biophysics
Volume33
Issue number1
StatePublished - Jan 2006

Keywords

  • 6q16.3-21
  • Childhood acute lymphoblastic leukemia
  • Digital differential display
  • Expressed sequence tags
  • Loss of heterozygosity
  • Tumor suppressor genes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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