Identification of genes regulated during mechanical load-induced cardiac hypertrophy

Sharon E. Johnatty, Jason R B Dyck, Lloyd H. Michael, Eric N. Olson, Maha Abdellatif

Research output: Contribution to journalArticlepeer-review

66 Scopus citations

Abstract

Cardiac hypertrophy is associated with both adaptive and adverse changes in gene expression. To identify genes regulated by pressure overload, we performed suppressive subtractive hybridization between cDNA from the hearts of aortic-banded (7-day) and sham-operated mice. In parallel, we performed a subtraction between an adult and a neonatal heart, for the purpose of comparing different forms of cardiac hypertrophy. Sequencing more than 100 clones led to the identification of an array of functionally known (70%) and unknown genes (30%) that are upregulated during cardiac growth. At least nine of those genes were preferentially expressed in both the neonatal and pressure over-load hearts alike. Using Northern blot analysis to investigate whether some of the identified genes were upregulated in the load-independent calcineurin-induced cardiac hypertrophy mouse model, revealed its incomplete similarity with the former models of cardiac growth. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)805-815
Number of pages11
JournalJournal of Molecular and Cellular Cardiology
Volume32
Issue number5
DOIs
StatePublished - Jan 1 2000

Keywords

  • Calcineurin
  • Cardiac hypertrophy
  • Gene induction
  • Pressure overload
  • Subtractive hybridization

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

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