Identification, purification, and characterization of a protein activator (PA28) of the 20 S proteasome (macropain)

Ma Chu-Ping, Clive A. Slaughter, George N. DeMartino

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Abstract

A protein that greatly stimulates the multiple pepti-dase activities of the 20 S proteasome (also known as macropain, the multicatalytic protease complex, and 20 S protease) has been purified from bovine red blood cells and from bovine heart. The activator protein was a single polypeptide with an apparent molecular weight of 28,000, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and had a native molecular weight of approximately 180,000. This protein, which we have termed PA28, regulated all three of the putatively distinct peptidase activities displayed by each of two functionally different forms of the proteasome. This regulation usually included both an increase in the maximal reaction velocity and a decrease in the concentration of substrate required for half-maximal velocity and indicated that PA28 acted as a positive allosteric effector of the proteasome. PA28 failed, however, to stimulate the hydrolysis of large protein substrates such as casein and lysozyme. These results suggested that the hydrolysis of protein substrates occurred at a site or sites distinct from those that hydrolyzed small peptides and that the regulation of the two processes could be uncoupled. Evidence for direct binding of PA28 to the proteasome was obtained by glycerol density gradient centrifugation. PA28 may play an important regulatory role in intracellular proteolytic pathways mediated by the proteasome.

Original languageEnglish (US)
Pages (from-to)10515-10523
Number of pages9
JournalJournal of Biological Chemistry
Volume267
Issue number15
StatePublished - May 25 1992

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Proteasome Endopeptidase Complex
Purification
Proteins
Hydrolysis
Peptide Hydrolases
Substrates
Molecular Weight
Molecular weight
Peptides
Density Gradient Centrifugation
Centrifugation
Muramidase
Caseins
Electrophoresis
Sodium Dodecyl Sulfate
Glycerol
Polyacrylamide Gel Electrophoresis
Blood
Erythrocytes
Cells

ASJC Scopus subject areas

  • Biochemistry

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Identification, purification, and characterization of a protein activator (PA28) of the 20 S proteasome (macropain). / Chu-Ping, Ma; Slaughter, Clive A.; DeMartino, George N.

In: Journal of Biological Chemistry, Vol. 267, No. 15, 25.05.1992, p. 10515-10523.

Research output: Contribution to journalArticle

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