Identity of Treponema pallidum subsp. pallidum polypeptides: Correlation of sodium dodecyl sulfate‐polyacrylamide gel electrophoresis results from different laboratories

Steven J. Norris, John F. Alderete, Nils H. Axelsen, Mark J. Bailey, Sharon A. Baker‐Zander, Joel B. Baseman, Philip J. Bassford, Robert E. Baughn, Alan Cockayne, Philip A. Hanff, Peter Hindersson, Sandra A. Larsen, Michael A. Lovett, Sheila A. Lukehart, James N. Miller, Matthäus A. Moskophidis, Ferdinand Müller, Michael V. Norgard, Charles W. Penn, Lola V. StammJan D. van Embden, Konrad Wicher

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

As the first step in a cooperative effort to standardize the identification of the polypeptides of Treponema pallidum subsp. pallidum, the sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) results obtained in 16 laboratories were compared. Although it was possible to correlate the positions of 16 of the major polypeptide bands, the cross‐identification of many of the polypeptides was ambiguous, particularly in the low molecular weight range. Two‐dimensional electrophoresis provided an improved means of separating and characterizing T. pallidum polypeptides as isolated molecular species. An approach to the unambiguous identification of treponemal polypeptides was outlined which will utilize two‐dimensional electrophoresis in combination with specific properties attributable to individual proteins, including reactivity with monoclonal antibodies or monospecific antisera, biochemical and structural properties, and sequence information. To demonstrate the feasibility of this approach, two‐dimensional electrophoresis in conjunction with immunoperoxidase staining was used to specifically identify three cloned T. pallidum proteins.

Original languageEnglish (US)
Pages (from-to)77-92
Number of pages16
JournalELECTROPHORESIS
Volume8
Issue number2
DOIs
StatePublished - 1987

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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    Norris, S. J., Alderete, J. F., Axelsen, N. H., Bailey, M. J., Baker‐Zander, S. A., Baseman, J. B., Bassford, P. J., Baughn, R. E., Cockayne, A., Hanff, P. A., Hindersson, P., Larsen, S. A., Lovett, M. A., Lukehart, S. A., Miller, J. N., Moskophidis, M. A., Müller, F., Norgard, M. V., Penn, C. W., ... Wicher, K. (1987). Identity of Treponema pallidum subsp. pallidum polypeptides: Correlation of sodium dodecyl sulfate‐polyacrylamide gel electrophoresis results from different laboratories. ELECTROPHORESIS, 8(2), 77-92. https://doi.org/10.1002/elps.1150080202