TY - JOUR
T1 - Imaging of post-mortem human brain tissue using electron and X-ray microscopy
AU - Lewis, Amanda J.
AU - Genoud, Christel
AU - Pont, Mélissa
AU - van de Berg, Wilma DJ
AU - Frank, Stephan
AU - Stahlberg, Henning
AU - Shahmoradian, Sarah H.
AU - Al-Amoudi, Ashraf
N1 - Funding Information:
We thank Liliana Malinovska and Paula Picotti for providing the yeast cells overexpressing GFP-tagged alpha synuclein, Kenneth N. Goldie and Ariane Fecteau-LeFebvre for EM support, and the Netherlands Brain Bank for providing the PMHB tissue samples. This work was in part supported by the Swiss National Science Foundation, grant CRSII5_177195/1, the Synapsis Foundation, the Heidi Seiler Stiftung, and the NCCR TransCure.
Funding Information:
We thank Liliana Malinovska and Paula Picotti for providing the yeast cells overexpressing GFP-tagged alpha synuclein, Kenneth N. Goldie and Ariane Fecteau-LeFebvre for EM support, and the Netherlands Brain Bank for providing the PMHB tissue samples. This work was in part supported by the Swiss National Science Foundation , grant CRSII5_177195/1 , the Synapsis Foundation , the Heidi Seiler Stiftung , and the NCCR TransCure.
Publisher Copyright:
© 2019 The Authors
PY - 2019/10
Y1 - 2019/10
N2 - Electron microscopy imaging of post-mortem human brain (PMHB) comes with a unique set of challenges due to numerous parameters beyond the researcher's control. Nevertheless, the wealth of information provided by the ultrastructural analysis of PMHB is proving crucial in our understanding of neurodegenerative diseases. This review highlights the importance of such studies and covers challenges, limitations and recent developments in the application of current EM imaging, including cryo-ET and correlative hybrid techniques, on PMHB.
AB - Electron microscopy imaging of post-mortem human brain (PMHB) comes with a unique set of challenges due to numerous parameters beyond the researcher's control. Nevertheless, the wealth of information provided by the ultrastructural analysis of PMHB is proving crucial in our understanding of neurodegenerative diseases. This review highlights the importance of such studies and covers challenges, limitations and recent developments in the application of current EM imaging, including cryo-ET and correlative hybrid techniques, on PMHB.
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U2 - 10.1016/j.sbi.2019.06.003
DO - 10.1016/j.sbi.2019.06.003
M3 - Review article
C2 - 31349127
AN - SCOPUS:85069749239
SN - 0959-440X
VL - 58
SP - 138
EP - 148
JO - Current Opinion in Structural Biology
JF - Current Opinion in Structural Biology
ER -