@article{3bf2c9ca4952483cbd3157493566fe8a,
title = "Immune Checkpoint Ligand Bioengineered Schwann Cells as Antigen-Specific Therapy for Experimental Autoimmune Encephalomyelitis",
abstract = "Failure to establish immune tolerance leads to the development of autoimmune disease. The ability to regulate autoreactive T cells without inducing systemic immunosuppression represents a major challenge in the development of new strategies to treat autoimmune disease. Here, a translational method for bioengineering programmed death-ligand 1 (PD-L1)- and cluster of differentiation 86 (CD86)-functionalized mouse Schwann cells (SCs) to prevent and ameliorate multiple sclerosis (MS) in established mouse models of chronic and relapsing-remitting experimental autoimmune encephalomyelitis (EAE) is described. It is shown that the intravenous (i.v.) administration of immune checkpoint ligand functionalized mouse SCs modifies the course of disease and ameliorates EAE. Further, it is found that such bioengineered mouse SCs inhibit the differentiation of myelin-specific helper T cells into pathogenic T helper type-1 (Th1) and type-17 (Th17) cells, promote the development of tolerogenic myelin-specific regulatory T (Treg) cells, and resolve inflammatory central nervous system microenvironments without inducing systemic immunosuppression.",
author = "Au, {Kin Man} and Roland Tisch and Wang, {Andrew Z.}",
note = "Funding Information: The authors thank the Microscopy Service Laboratory Core, Animal Study Core, Small Animal Imaging Facility, Animal Clinical Laboratory, Animal Histopathology Core Facility, Translation Pathology Lab, UNC Flow Cytometry Core Facility, UNC Macromolecular Interactions Facility and UNC Michael Hooker Proteomics Centre in the School of Medicine, and Chapel Hill Analytical and Nanofabrication Laboratory (CHANL) at the University of North Carolina at Chapel Hill for their assistance with procedures in this manuscript. UNC Lineberger Animal Studies Core is supported in part by an NCI Center Core Support Grant (CA16086) to the UNC Lineberger Comprehensive Cancer Center. The UNC Flow Cytometry Core Facility is supported in part by P30CA016086 Cancer Center Core Support Grant to the UNC Lineberger Comprehensive Cancer Center. This work was supported by the University Cancer Research Fund from the University of North Carolina and R01CA178748 grant from the National Institutes of Health/National Cancer Institute. A.Z.W. was also supported by the National Institutes of Health Center for Nanotechnology Excellence grant U54-CA151652. R.T. was supported by National Institutes of Health/NIAID grants R01AI139475 and R01AI141631. All procedures involving the experimental animals in this work were performed following protocols that were approved by the University of North Carolina Institutional Animal Care and Use Committee and they conformed with the Guide for the Care and Use of Laboratory Animals (NIH publication no. 86-23, revised 1985). Funding Information: The authors thank the Microscopy Service Laboratory Core, Animal Study Core, Small Animal Imaging Facility, Animal Clinical Laboratory, Animal Histopathology Core Facility, Translation Pathology Lab, UNC Flow Cytometry Core Facility, UNC Macromolecular Interactions Facility and UNC Michael Hooker Proteomics Centre in the School of Medicine, and Chapel Hill Analytical and Nanofabrication Laboratory (CHANL) at the University of North Carolina at Chapel Hill for their assistance with procedures in this manuscript. UNC Lineberger Animal Studies Core is supported in part by an NCI Center Core Support Grant (CA16086) to the UNC Lineberger Comprehensive Cancer Center. The UNC Flow Cytometry Core Facility is supported in part by P30CA016086 Cancer Center Core Support Grant to the UNC Lineberger Comprehensive Cancer Center. This work was supported by the University Cancer Research Fund from the University of North Carolina and R01CA178748 grant from the National Institutes of Health/National Cancer Institute. A.Z.W. was also supported by the National Institutes of Health Center for Nanotechnology Excellence grant U54‐CA151652. R.T. was supported by National Institutes of Health/NIAID grants R01AI139475 and R01AI141631. All procedures involving the experimental animals in this work were performed following protocols that were approved by the University of North Carolina Institutional Animal Care and Use Committee and they conformed with the Guide for the Care and Use of Laboratory Animals (NIH publication no. 86‐23, revised 1985). Publisher Copyright: {\textcopyright} 2021 Wiley-VCH GmbH",
year = "2022",
month = feb,
day = "3",
doi = "10.1002/adma.202107392",
language = "English (US)",
volume = "34",
journal = "Advanced Materials",
issn = "0935-9648",
publisher = "Wiley-VCH Verlag",
number = "5",
}