Immunoexpression of SALL4 in Wilms tumors and developing kidney

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

SALL4 is a zinc finger transcription factor that plays a role in the maintainence and pluripotency of embryonic stem cell and is important in renal development where SALL4 mutations give rise to renal malformations. Because Wilms tumor recapitulates renal embryogenesis, we hypothesized that Wilms tumor cells may also express SALL4. We performed immunohistochemistry for SALL4 on tissue microarray sections of Wilms tumors, nephrogenic rests, and fetal renal cortices. Half (26 out of 52) of the Wilms tumors showed SALL4 immunoreactivity, ranging from strong and diffuse to focal and weak. Blastemal, epithelial, and combined blastemal and epithelial patterns of immunoreactivity were identified. No cases showed stromal staining. In the fetal kidney, SALL4 expression was restricted to the blastema and primitive epithelium at 15 weeks' gestation. SALL4 staining was not seen at later gestational ages, in non-neoplastic postnatal kidneys, or in nephrogenic rests. Our study is the first to demonstrate SALL4 immunoreactivity in Wilms tumors and in developing fetal kidney. The absence of SALL4 staining in nephrogenic rests, the presumed precursors of Wilms tumors, is intriguing and suggests that Wilms tumors have a pluripotency quality that may be lacking in nephrogenic rests.

Original languageEnglish (US)
Pages (from-to)639-644
Number of pages6
JournalPathology and Oncology Research
Volume17
Issue number3
DOIs
StatePublished - Sep 2011

Fingerprint

Wilms Tumor
Kidney
Staining and Labeling
Zinc Fingers
Embryonic Stem Cells
Gestational Age
Embryonic Development
Transcription Factors
Epithelium
Immunohistochemistry
Pregnancy
Mutation

Keywords

  • Immunohistochemistry
  • Kidney development
  • Nephrogenic rest
  • SALL4
  • Wilms tumor

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Pathology and Forensic Medicine

Cite this

Immunoexpression of SALL4 in Wilms tumors and developing kidney. / Deisch, Jeremy; Raisanen, Jack M; Rakheja, Dinesh.

In: Pathology and Oncology Research, Vol. 17, No. 3, 09.2011, p. 639-644.

Research output: Contribution to journalArticle

@article{dcdc2c340d1c408599a9dea663405c65,
title = "Immunoexpression of SALL4 in Wilms tumors and developing kidney",
abstract = "SALL4 is a zinc finger transcription factor that plays a role in the maintainence and pluripotency of embryonic stem cell and is important in renal development where SALL4 mutations give rise to renal malformations. Because Wilms tumor recapitulates renal embryogenesis, we hypothesized that Wilms tumor cells may also express SALL4. We performed immunohistochemistry for SALL4 on tissue microarray sections of Wilms tumors, nephrogenic rests, and fetal renal cortices. Half (26 out of 52) of the Wilms tumors showed SALL4 immunoreactivity, ranging from strong and diffuse to focal and weak. Blastemal, epithelial, and combined blastemal and epithelial patterns of immunoreactivity were identified. No cases showed stromal staining. In the fetal kidney, SALL4 expression was restricted to the blastema and primitive epithelium at 15 weeks' gestation. SALL4 staining was not seen at later gestational ages, in non-neoplastic postnatal kidneys, or in nephrogenic rests. Our study is the first to demonstrate SALL4 immunoreactivity in Wilms tumors and in developing fetal kidney. The absence of SALL4 staining in nephrogenic rests, the presumed precursors of Wilms tumors, is intriguing and suggests that Wilms tumors have a pluripotency quality that may be lacking in nephrogenic rests.",
keywords = "Immunohistochemistry, Kidney development, Nephrogenic rest, SALL4, Wilms tumor",
author = "Jeremy Deisch and Raisanen, {Jack M} and Dinesh Rakheja",
year = "2011",
month = "9",
doi = "10.1007/s12253-011-9364-0",
language = "English (US)",
volume = "17",
pages = "639--644",
journal = "Pathology and Oncology Research",
issn = "1219-4956",
publisher = "Springer Netherlands",
number = "3",

}

TY - JOUR

T1 - Immunoexpression of SALL4 in Wilms tumors and developing kidney

AU - Deisch, Jeremy

AU - Raisanen, Jack M

AU - Rakheja, Dinesh

PY - 2011/9

Y1 - 2011/9

N2 - SALL4 is a zinc finger transcription factor that plays a role in the maintainence and pluripotency of embryonic stem cell and is important in renal development where SALL4 mutations give rise to renal malformations. Because Wilms tumor recapitulates renal embryogenesis, we hypothesized that Wilms tumor cells may also express SALL4. We performed immunohistochemistry for SALL4 on tissue microarray sections of Wilms tumors, nephrogenic rests, and fetal renal cortices. Half (26 out of 52) of the Wilms tumors showed SALL4 immunoreactivity, ranging from strong and diffuse to focal and weak. Blastemal, epithelial, and combined blastemal and epithelial patterns of immunoreactivity were identified. No cases showed stromal staining. In the fetal kidney, SALL4 expression was restricted to the blastema and primitive epithelium at 15 weeks' gestation. SALL4 staining was not seen at later gestational ages, in non-neoplastic postnatal kidneys, or in nephrogenic rests. Our study is the first to demonstrate SALL4 immunoreactivity in Wilms tumors and in developing fetal kidney. The absence of SALL4 staining in nephrogenic rests, the presumed precursors of Wilms tumors, is intriguing and suggests that Wilms tumors have a pluripotency quality that may be lacking in nephrogenic rests.

AB - SALL4 is a zinc finger transcription factor that plays a role in the maintainence and pluripotency of embryonic stem cell and is important in renal development where SALL4 mutations give rise to renal malformations. Because Wilms tumor recapitulates renal embryogenesis, we hypothesized that Wilms tumor cells may also express SALL4. We performed immunohistochemistry for SALL4 on tissue microarray sections of Wilms tumors, nephrogenic rests, and fetal renal cortices. Half (26 out of 52) of the Wilms tumors showed SALL4 immunoreactivity, ranging from strong and diffuse to focal and weak. Blastemal, epithelial, and combined blastemal and epithelial patterns of immunoreactivity were identified. No cases showed stromal staining. In the fetal kidney, SALL4 expression was restricted to the blastema and primitive epithelium at 15 weeks' gestation. SALL4 staining was not seen at later gestational ages, in non-neoplastic postnatal kidneys, or in nephrogenic rests. Our study is the first to demonstrate SALL4 immunoreactivity in Wilms tumors and in developing fetal kidney. The absence of SALL4 staining in nephrogenic rests, the presumed precursors of Wilms tumors, is intriguing and suggests that Wilms tumors have a pluripotency quality that may be lacking in nephrogenic rests.

KW - Immunohistochemistry

KW - Kidney development

KW - Nephrogenic rest

KW - SALL4

KW - Wilms tumor

UR - http://www.scopus.com/inward/record.url?scp=80052760824&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80052760824&partnerID=8YFLogxK

U2 - 10.1007/s12253-011-9364-0

DO - 10.1007/s12253-011-9364-0

M3 - Article

C2 - 21258884

AN - SCOPUS:80052760824

VL - 17

SP - 639

EP - 644

JO - Pathology and Oncology Research

JF - Pathology and Oncology Research

SN - 1219-4956

IS - 3

ER -