Immunologic characterization of tumor markers in human ovarian cancer cell lines

William H. Kutteh, David Scott Miller, J. Michael Mathis

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

OBJECTIVE: The purpose of this study was to evaluate the expression of a novel autologous ovarian tumor-associated antigen in eight human ovarian tumor cell lines compared with other ovarian tumor markers and products of oncogenes. METHODS: Autologous antibodies were eluted from human ovarian tumor-membrane fragments purified in our laboratory. These antibodies react with autologous ovarian tumor-associated antigens (AOTA) and have a high degree of specificity for human ovarian tumors. They do not bind to normal ovarian or nonovarian tissues, or to nonovarian neoplastic tissues. We evaluated eight human ovarian adenocarcinoma cell lines (2008, 2774, Caov-3, OI'CAR-3, PA-1, SW 626, UCI 101, and UCI 107) by indirect immunofluorescence to determine the expression of AOTA relative to the ovarian cancer tumor marker CA 125 and the products of selected oncogenes (p 53, c-neu, and c- myc). RESULTS: The patterns ana intensities of immunofluorescence correlated most closely between AOTA and c-neu. For example, AOTA and c-neu were detected in all eight cell lines and displayed very strong cytoplasmic fluorescence on cell lines 2774, UCI 101, and UCI 107. CA 125 was present in the cytoplasm of four of eight cell lines. A tumor suppressor gene product, p53, exhibited a nuclear staining pattern in six of eight cell lines. CONCLUSIONS: These data suggest that AOTA and the products of the c-neu oncogene may share certain epitopes. Current studies are underway to increase our understanding of the humoral response to ovarian cancer and the possible relationship to the expression of tumor oncogene products. Further characterization of AOTA will be necessary before early diagnostic tests can be developed.

Original languageEnglish (US)
Pages (from-to)216-222
Number of pages7
JournalJournal of the Society for Gynecologic Investigation
Volume3
Issue number4
DOIs
StatePublished - Jul 1996

Fingerprint

Tumor Biomarkers
Ovarian Neoplasms
Cell Line
Oncogene Proteins
Neoplasms
Antibodies
Indirect Fluorescent Antibody Technique
ovarian tumor associated antigen
Tumor Cell Line
Tumor Suppressor Genes
Oncogenes
Routine Diagnostic Tests
Fluorescent Antibody Technique
Epitopes
Cytoplasm
Adenocarcinoma
Fluorescence
Staining and Labeling
Membranes

Keywords

  • autologous antibodies
  • c-myc
  • c-neu
  • ovarian neoplasia
  • Tumor markers

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Immunologic characterization of tumor markers in human ovarian cancer cell lines. / Kutteh, William H.; Miller, David Scott; Mathis, J. Michael.

In: Journal of the Society for Gynecologic Investigation, Vol. 3, No. 4, 07.1996, p. 216-222.

Research output: Contribution to journalArticle

@article{983984998ea142058b93baa28379c370,
title = "Immunologic characterization of tumor markers in human ovarian cancer cell lines",
abstract = "OBJECTIVE: The purpose of this study was to evaluate the expression of a novel autologous ovarian tumor-associated antigen in eight human ovarian tumor cell lines compared with other ovarian tumor markers and products of oncogenes. METHODS: Autologous antibodies were eluted from human ovarian tumor-membrane fragments purified in our laboratory. These antibodies react with autologous ovarian tumor-associated antigens (AOTA) and have a high degree of specificity for human ovarian tumors. They do not bind to normal ovarian or nonovarian tissues, or to nonovarian neoplastic tissues. We evaluated eight human ovarian adenocarcinoma cell lines (2008, 2774, Caov-3, OI'CAR-3, PA-1, SW 626, UCI 101, and UCI 107) by indirect immunofluorescence to determine the expression of AOTA relative to the ovarian cancer tumor marker CA 125 and the products of selected oncogenes (p 53, c-neu, and c- myc). RESULTS: The patterns ana intensities of immunofluorescence correlated most closely between AOTA and c-neu. For example, AOTA and c-neu were detected in all eight cell lines and displayed very strong cytoplasmic fluorescence on cell lines 2774, UCI 101, and UCI 107. CA 125 was present in the cytoplasm of four of eight cell lines. A tumor suppressor gene product, p53, exhibited a nuclear staining pattern in six of eight cell lines. CONCLUSIONS: These data suggest that AOTA and the products of the c-neu oncogene may share certain epitopes. Current studies are underway to increase our understanding of the humoral response to ovarian cancer and the possible relationship to the expression of tumor oncogene products. Further characterization of AOTA will be necessary before early diagnostic tests can be developed.",
keywords = "autologous antibodies, c-myc, c-neu, ovarian neoplasia, Tumor markers",
author = "Kutteh, {William H.} and Miller, {David Scott} and Mathis, {J. Michael}",
year = "1996",
month = "7",
doi = "10.1016/1071-5576(96)00013-5",
language = "English (US)",
volume = "3",
pages = "216--222",
journal = "Reproductive Sciences",
issn = "1933-7191",
publisher = "SAGE Publications Inc.",
number = "4",

}

TY - JOUR

T1 - Immunologic characterization of tumor markers in human ovarian cancer cell lines

AU - Kutteh, William H.

AU - Miller, David Scott

AU - Mathis, J. Michael

PY - 1996/7

Y1 - 1996/7

N2 - OBJECTIVE: The purpose of this study was to evaluate the expression of a novel autologous ovarian tumor-associated antigen in eight human ovarian tumor cell lines compared with other ovarian tumor markers and products of oncogenes. METHODS: Autologous antibodies were eluted from human ovarian tumor-membrane fragments purified in our laboratory. These antibodies react with autologous ovarian tumor-associated antigens (AOTA) and have a high degree of specificity for human ovarian tumors. They do not bind to normal ovarian or nonovarian tissues, or to nonovarian neoplastic tissues. We evaluated eight human ovarian adenocarcinoma cell lines (2008, 2774, Caov-3, OI'CAR-3, PA-1, SW 626, UCI 101, and UCI 107) by indirect immunofluorescence to determine the expression of AOTA relative to the ovarian cancer tumor marker CA 125 and the products of selected oncogenes (p 53, c-neu, and c- myc). RESULTS: The patterns ana intensities of immunofluorescence correlated most closely between AOTA and c-neu. For example, AOTA and c-neu were detected in all eight cell lines and displayed very strong cytoplasmic fluorescence on cell lines 2774, UCI 101, and UCI 107. CA 125 was present in the cytoplasm of four of eight cell lines. A tumor suppressor gene product, p53, exhibited a nuclear staining pattern in six of eight cell lines. CONCLUSIONS: These data suggest that AOTA and the products of the c-neu oncogene may share certain epitopes. Current studies are underway to increase our understanding of the humoral response to ovarian cancer and the possible relationship to the expression of tumor oncogene products. Further characterization of AOTA will be necessary before early diagnostic tests can be developed.

AB - OBJECTIVE: The purpose of this study was to evaluate the expression of a novel autologous ovarian tumor-associated antigen in eight human ovarian tumor cell lines compared with other ovarian tumor markers and products of oncogenes. METHODS: Autologous antibodies were eluted from human ovarian tumor-membrane fragments purified in our laboratory. These antibodies react with autologous ovarian tumor-associated antigens (AOTA) and have a high degree of specificity for human ovarian tumors. They do not bind to normal ovarian or nonovarian tissues, or to nonovarian neoplastic tissues. We evaluated eight human ovarian adenocarcinoma cell lines (2008, 2774, Caov-3, OI'CAR-3, PA-1, SW 626, UCI 101, and UCI 107) by indirect immunofluorescence to determine the expression of AOTA relative to the ovarian cancer tumor marker CA 125 and the products of selected oncogenes (p 53, c-neu, and c- myc). RESULTS: The patterns ana intensities of immunofluorescence correlated most closely between AOTA and c-neu. For example, AOTA and c-neu were detected in all eight cell lines and displayed very strong cytoplasmic fluorescence on cell lines 2774, UCI 101, and UCI 107. CA 125 was present in the cytoplasm of four of eight cell lines. A tumor suppressor gene product, p53, exhibited a nuclear staining pattern in six of eight cell lines. CONCLUSIONS: These data suggest that AOTA and the products of the c-neu oncogene may share certain epitopes. Current studies are underway to increase our understanding of the humoral response to ovarian cancer and the possible relationship to the expression of tumor oncogene products. Further characterization of AOTA will be necessary before early diagnostic tests can be developed.

KW - autologous antibodies

KW - c-myc

KW - c-neu

KW - ovarian neoplasia

KW - Tumor markers

UR - http://www.scopus.com/inward/record.url?scp=0030200559&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030200559&partnerID=8YFLogxK

U2 - 10.1016/1071-5576(96)00013-5

DO - 10.1016/1071-5576(96)00013-5

M3 - Article

C2 - 8796833

AN - SCOPUS:0030200559

VL - 3

SP - 216

EP - 222

JO - Reproductive Sciences

JF - Reproductive Sciences

SN - 1933-7191

IS - 4

ER -