Immunotoxins Containing Ricin A Or B Chains with Modified Carbohydrate Residues Act Synergistically in Killing Neoplastic B Cells In Vitro*

E. S. Vitetta, P. E. Thorpe

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Partially deglycosylated (dg) ricin A and B chains were coupled to rabbit anti-human Ig (IT-dg-A) and goat anti-rabbit Ig (IT-dg-B), respectively. The deglycosylation was accomplished by treating intact ricin (before separation into its constituent chains) with a mixture of sodium metaperiodate and sodium cyanobrohydride. The two immunotoxins (ITs) acted synergistically in vitro to kill Daudi cells, albeit to a four-to-eight-fold lesser degree than ITs prepared with native A and B chains.

Original languageEnglish (US)
Pages (from-to)191-198
Number of pages8
JournalCancer Drug Delivery
Volume2
Issue number3
DOIs
StatePublished - 1985

ASJC Scopus subject areas

  • Pharmacology
  • Cancer Research

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