Improvement of the precision in CFU-GM and BFU-E counting by flow cytometry-based standardization of short-term culture assays

S. Sheikhzadeh, H. J. Hammers, D. Hartwig, H. Kirchner, P. Schlenke

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The counting of colony-forming units granulocyte-macrophage (CFU-GM) and burst-forming units erythrocyte (BFU-E) provides substantial in vitro information about the graft quality after peripheral stem cell transplantation (PBSCT). By using different techniques for culturing and scoring, high inter- and intralaboratory coefficients of variation (CV) are frequently reported. We minimized the imprecision by using flow cytometry-based incorporation of constant numbers of CD34+ cells per culture dish instead of the formerly used mononuclear cells. Our results show acceptable CVs for CFU-GM (12.3%) and for BFU-E (13.3%) based on this seeding technique, which contributes to fulfilling the demands of a quality assurance system in stem cell laboratories.

Original languageEnglish (US)
Pages (from-to)881-885
Number of pages5
JournalJournal of Hematotherapy and Stem Cell Research
Volume10
Issue number6
DOIs
StatePublished - Dec 1 2001

ASJC Scopus subject areas

  • Immunology
  • Hematology

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