In vivo characterization of downfield peaks at 9.4 T: T2 relaxation times, quantification, pH estimation, and assignments

Tamas Borbath, Saipavitra Murali-Manohar, Andrew Martin Wright, Anke Henning

Research output: Contribution to journalArticle

Abstract

Purpose: Relaxation times are a valuable asset when determining spectral assignments. In this study, apparent T2 relaxation times ((Formula presented.)) of downfield peaks are reported in the human brain at 9.4 T and are used to guide spectral assignments of some downfield metabolite peaks. Methods: Echo time series of downfield metabolite spectra were acquired at 9.4 T using a metabolite-cycled semi-LASER sequence. Metabolite spectral fitting was performed using LCModel V6.3-1L while fitting a pH sweep to estimate the pH of the homocarnosine (hCs) imidazole ring. (Formula presented.) were calculated by fitting the resulting relative amplitudes of the peaks to a mono-exponential decay across the TE series. Furthermore, estimated tissue concentrations of molecules were calculated using the relaxation times and internal water as a reference. Results: (Formula presented.) of downfield metabolites are reported within a range from 16 to 32 ms except for homocarnosine with (Formula presented.) of 50 ms. Correcting (Formula presented.) for exchange rates ((Formula presented.)) resulted in relaxation times between 20 and 33 ms. The estimated pH values based on hCs imidazole range from 7.07 to 7.12 between subjects. Furthermore, analyzing the linewidths of the downfield peaks and their (Formula presented.) contribution led to possible peak assignments. Conclusion: (Formula presented.) relaxation times were longer for the assigned metabolite peaks compared to the unassigned peaks. Tissue pH estimation in vivo with proton MRS and simultaneous quantification of amide protons at 8.30 ± 0.15 ppm is likely possible. Based on concentration, linewidth, and exchange rates measurements, tentative peak assignments are discussed for adenosine triphosphate (ATP), N-acetylaspartylglutamate (NAAG), and urea.

Original languageEnglish (US)
JournalMagnetic resonance in medicine
DOIs
StateAccepted/In press - 2020

Keywords

  • downfield
  • exchange rates
  • MR spectroscopy
  • pH
  • quantification
  • T relaxation time
  • ultra-high field

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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