Inability of choleragenoid sensitized cells to induce T cell mediated cytolysis

J. Forman, R. A. Finkelstein

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4 Scopus citations

Abstract

Murine splenocytes and tumor cells bind cholera enterotoxoid (choleragenoid). 4 hours after sensitization, choleragenoid-coated cells were lysed in the presence of anti-choleragenoid serum and complement, indicating that the binding was stable. Choleragenoid-coated cells were unable to sensitize spleen cells from normal or choleragenoid primed syngeneic mice into displaying a cytotoxic effect against choleragenoid-coated target cells in the T cell-mediated cytotoxicity assay. Cells coated with both choleragenoid and trinitrophenyl (TNP) groups did sensitize syngeneic spleen cells to display a cytotoxic effect against target cells bearing choleragenoid and TNP or TNP alone, but not choleragenoid alone. The data demonstrate that the mere binding of a foreign component to lymphoid cells is not sufficient to allow sensitization of cytotoxic T cells.

Original languageEnglish (US)
Pages (from-to)1655-1658
Number of pages4
JournalJournal of Immunology
Volume118
Issue number5
Publication statusPublished - Jan 1 1977

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ASJC Scopus subject areas

  • Immunology

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