Murine splenocytes and tumor cells bind cholera enterotoxoid (choleragenoid). 4 hours after sensitization, choleragenoid-coated cells were lysed in the presence of anti-choleragenoid serum and complement, indicating that the binding was stable. Choleragenoid-coated cells were unable to sensitize spleen cells from normal or choleragenoid primed syngeneic mice into displaying a cytotoxic effect against choleragenoid-coated target cells in the T cell-mediated cytotoxicity assay. Cells coated with both choleragenoid and trinitrophenyl (TNP) groups did sensitize syngeneic spleen cells to display a cytotoxic effect against target cells bearing choleragenoid and TNP or TNP alone, but not choleragenoid alone. The data demonstrate that the mere binding of a foreign component to lymphoid cells is not sufficient to allow sensitization of cytotoxic T cells.
|Original language||English (US)|
|Number of pages||4|
|Journal||Journal of Immunology|
|State||Published - Jan 1 1977|
ASJC Scopus subject areas
- Immunology and Allergy