Increased DJ-1 expression under oxidative stress and in Alzheimer's disease brains

Stéphanie Baulac, Hope Lu, Jennifer Strahle, Ting Yang, Matthew S. Goldberg, Jie Shen, Michael G. Schlossmacher, Cynthia A. Lemere, Qun Lu, Weiming Xia

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Mutations in the DJ-1 gene have been linked to autosomal recessive familial Parkinson's disease. To understand the function of DJ-1, we determined the DJ-1 expression in both zebrafish and post mortem human brains. We found that DJ-1 was expressed early during zebrafish development and throughout adulthood. Knock down (KD) of DJ-1 by injection of morpholino did not cause dramatic morphologic alterations during development, and no loss of dopaminergic neurons was observed in embryos lacking DJ-1. However, DJ-1 KD embryos were more susceptible to programmed cell death. While a slight reduction in staining for islet-1 positive neurons was observed in both DJ-1 KD and H2O 2treated embryos, the number of apoptotic cells was significantly increased in both KD and H2O2treated embryos. Interestingly, DJ-1 expression was increased in brains of zebrafish under conditions of oxidative stress, indicating that DJ-1 is a part of stress-responsive machinery. Since oxidative stress is one of the major contributors to the development of Alzheimer's disease (AD), we also examined DJ-1 expression in AD brains. Using DJ-1 specific antibodies, we failed to detect a robust staining of DJ-1 in brain tissues from control subjects. However, DJ-1 immunoreactivity was detected in hippocampal pyramidal neurons and astrocytes of AD brains. Therefore, our results strongly suggest that DJ-1 expression is not necessary during zebrafish development but can be induced in zebrafish exposed to oxidative stress and is present in human AD brains.

Original languageEnglish (US)
Article number12
JournalMolecular Neurodegeneration
Volume4
Issue number1
DOIs
StatePublished - Mar 18 2009
Externally publishedYes

Fingerprint

Zebrafish
Alzheimer Disease
Oxidative Stress
Brain
Embryonic Structures
Staining and Labeling
Morpholinos
Pyramidal Cells
Dopaminergic Neurons
Parkinsonian Disorders
Astrocytes
Cell Death
Cell Count
Neurons
Mutation
Injections
Antibodies
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

Cite this

Increased DJ-1 expression under oxidative stress and in Alzheimer's disease brains. / Baulac, Stéphanie; Lu, Hope; Strahle, Jennifer; Yang, Ting; Goldberg, Matthew S.; Shen, Jie; Schlossmacher, Michael G.; Lemere, Cynthia A.; Lu, Qun; Xia, Weiming.

In: Molecular Neurodegeneration, Vol. 4, No. 1, 12, 18.03.2009.

Research output: Contribution to journalArticle

Baulac, S, Lu, H, Strahle, J, Yang, T, Goldberg, MS, Shen, J, Schlossmacher, MG, Lemere, CA, Lu, Q & Xia, W 2009, 'Increased DJ-1 expression under oxidative stress and in Alzheimer's disease brains', Molecular Neurodegeneration, vol. 4, no. 1, 12. https://doi.org/10.1186/1750-1326-4-12
Baulac, Stéphanie ; Lu, Hope ; Strahle, Jennifer ; Yang, Ting ; Goldberg, Matthew S. ; Shen, Jie ; Schlossmacher, Michael G. ; Lemere, Cynthia A. ; Lu, Qun ; Xia, Weiming. / Increased DJ-1 expression under oxidative stress and in Alzheimer's disease brains. In: Molecular Neurodegeneration. 2009 ; Vol. 4, No. 1.
@article{9575797a9ebe48daac6796687a949d64,
title = "Increased DJ-1 expression under oxidative stress and in Alzheimer's disease brains",
abstract = "Mutations in the DJ-1 gene have been linked to autosomal recessive familial Parkinson's disease. To understand the function of DJ-1, we determined the DJ-1 expression in both zebrafish and post mortem human brains. We found that DJ-1 was expressed early during zebrafish development and throughout adulthood. Knock down (KD) of DJ-1 by injection of morpholino did not cause dramatic morphologic alterations during development, and no loss of dopaminergic neurons was observed in embryos lacking DJ-1. However, DJ-1 KD embryos were more susceptible to programmed cell death. While a slight reduction in staining for islet-1 positive neurons was observed in both DJ-1 KD and H2O 2treated embryos, the number of apoptotic cells was significantly increased in both KD and H2O2treated embryos. Interestingly, DJ-1 expression was increased in brains of zebrafish under conditions of oxidative stress, indicating that DJ-1 is a part of stress-responsive machinery. Since oxidative stress is one of the major contributors to the development of Alzheimer's disease (AD), we also examined DJ-1 expression in AD brains. Using DJ-1 specific antibodies, we failed to detect a robust staining of DJ-1 in brain tissues from control subjects. However, DJ-1 immunoreactivity was detected in hippocampal pyramidal neurons and astrocytes of AD brains. Therefore, our results strongly suggest that DJ-1 expression is not necessary during zebrafish development but can be induced in zebrafish exposed to oxidative stress and is present in human AD brains.",
author = "St{\'e}phanie Baulac and Hope Lu and Jennifer Strahle and Ting Yang and Goldberg, {Matthew S.} and Jie Shen and Schlossmacher, {Michael G.} and Lemere, {Cynthia A.} and Qun Lu and Weiming Xia",
year = "2009",
month = "3",
day = "18",
doi = "10.1186/1750-1326-4-12",
language = "English (US)",
volume = "4",
journal = "Molecular Neurodegeneration",
issn = "1750-1326",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - Increased DJ-1 expression under oxidative stress and in Alzheimer's disease brains

AU - Baulac, Stéphanie

AU - Lu, Hope

AU - Strahle, Jennifer

AU - Yang, Ting

AU - Goldberg, Matthew S.

AU - Shen, Jie

AU - Schlossmacher, Michael G.

AU - Lemere, Cynthia A.

AU - Lu, Qun

AU - Xia, Weiming

PY - 2009/3/18

Y1 - 2009/3/18

N2 - Mutations in the DJ-1 gene have been linked to autosomal recessive familial Parkinson's disease. To understand the function of DJ-1, we determined the DJ-1 expression in both zebrafish and post mortem human brains. We found that DJ-1 was expressed early during zebrafish development and throughout adulthood. Knock down (KD) of DJ-1 by injection of morpholino did not cause dramatic morphologic alterations during development, and no loss of dopaminergic neurons was observed in embryos lacking DJ-1. However, DJ-1 KD embryos were more susceptible to programmed cell death. While a slight reduction in staining for islet-1 positive neurons was observed in both DJ-1 KD and H2O 2treated embryos, the number of apoptotic cells was significantly increased in both KD and H2O2treated embryos. Interestingly, DJ-1 expression was increased in brains of zebrafish under conditions of oxidative stress, indicating that DJ-1 is a part of stress-responsive machinery. Since oxidative stress is one of the major contributors to the development of Alzheimer's disease (AD), we also examined DJ-1 expression in AD brains. Using DJ-1 specific antibodies, we failed to detect a robust staining of DJ-1 in brain tissues from control subjects. However, DJ-1 immunoreactivity was detected in hippocampal pyramidal neurons and astrocytes of AD brains. Therefore, our results strongly suggest that DJ-1 expression is not necessary during zebrafish development but can be induced in zebrafish exposed to oxidative stress and is present in human AD brains.

AB - Mutations in the DJ-1 gene have been linked to autosomal recessive familial Parkinson's disease. To understand the function of DJ-1, we determined the DJ-1 expression in both zebrafish and post mortem human brains. We found that DJ-1 was expressed early during zebrafish development and throughout adulthood. Knock down (KD) of DJ-1 by injection of morpholino did not cause dramatic morphologic alterations during development, and no loss of dopaminergic neurons was observed in embryos lacking DJ-1. However, DJ-1 KD embryos were more susceptible to programmed cell death. While a slight reduction in staining for islet-1 positive neurons was observed in both DJ-1 KD and H2O 2treated embryos, the number of apoptotic cells was significantly increased in both KD and H2O2treated embryos. Interestingly, DJ-1 expression was increased in brains of zebrafish under conditions of oxidative stress, indicating that DJ-1 is a part of stress-responsive machinery. Since oxidative stress is one of the major contributors to the development of Alzheimer's disease (AD), we also examined DJ-1 expression in AD brains. Using DJ-1 specific antibodies, we failed to detect a robust staining of DJ-1 in brain tissues from control subjects. However, DJ-1 immunoreactivity was detected in hippocampal pyramidal neurons and astrocytes of AD brains. Therefore, our results strongly suggest that DJ-1 expression is not necessary during zebrafish development but can be induced in zebrafish exposed to oxidative stress and is present in human AD brains.

UR - http://www.scopus.com/inward/record.url?scp=62149129981&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=62149129981&partnerID=8YFLogxK

U2 - 10.1186/1750-1326-4-12

DO - 10.1186/1750-1326-4-12

M3 - Article

C2 - 19243613

AN - SCOPUS:62149129981

VL - 4

JO - Molecular Neurodegeneration

JF - Molecular Neurodegeneration

SN - 1750-1326

IS - 1

M1 - 12

ER -