Induction by alkylating agents of sister chromatid exchanges and chromatid breaks in Fanconi's anemia

S. A. Latt, G. Stetten, L. A. Juergens, G. R. Buchanan, P. S. Gerald

Research output: Contribution to journalArticle

219 Citations (Scopus)

Abstract

Sister chromatid exchanges, which may reflect chromosome repair in response to certain types of DNA damage, provide a means of investigating the increased chromosome fragility characteristic of Fanconi's anemia. By a recently developed technique using 33258 Hoechst and 5 bromodeoxyuridine, it was observed that the baseline frequency of sister chromatid exchanges in phytohemagglutinin stimulated lymphocytes from four males with Fanconi's anemia differed little from that of normal lymphocytes. However, addition of the bifunctional alkylating agent mitomycin C (0.01 or 0.03 μg/ml) to the Fanconi's anemia cells during culture induces less than half of the increase in exchanges found in identically treated normal lymphocytes. This reduced increment in exchanges is accompanied by a partial suppression of mitosis and a marked increase in chromatid breaks and rearrangements. Many of these events occur at sites of incomplete chromatid interchange. The increase in sister chromatid exchanges induced in Fanconi's anemia lymphocytes by the monofunctional alkylating agent ethylmethane sulfonate (0.25 mg/ml) was slightly less than that in normal cells. Lymphocytes from two sets of parents of the patients with Fanconi's anemia exhibited a normal response to alkylating agents, while dermal fibroblasts from two different parients with Fanconi's anemia reacted to mitomycin C with an increase in chromatid breaks, but a nearly normal increment of sister chromatid exchanges. The results suggest that chromocomal breaks and rearrangements in Fanconi's anemia lymphocytes may result from a defect in a form of repair of DNA damage.

Original languageEnglish (US)
Pages (from-to)4066-4070
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume72
Issue number10
DOIs
StatePublished - 1975

Fingerprint

Fanconi Anemia
Sister Chromatid Exchange
Chromatids
Alkylating Agents
Lymphocytes
Mitomycin
DNA Damage
Chromosome Fragility
Ethyl Methanesulfonate
Bisbenzimidazole
Phytohemagglutinins
Bromodeoxyuridine
Mitosis
Cell Culture Techniques
Fibroblasts
Chromosomes
Parents
Skin

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Induction by alkylating agents of sister chromatid exchanges and chromatid breaks in Fanconi's anemia. / Latt, S. A.; Stetten, G.; Juergens, L. A.; Buchanan, G. R.; Gerald, P. S.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 72, No. 10, 1975, p. 4066-4070.

Research output: Contribution to journalArticle

@article{251e80d48943406ba26391eb08268678,
title = "Induction by alkylating agents of sister chromatid exchanges and chromatid breaks in Fanconi's anemia",
abstract = "Sister chromatid exchanges, which may reflect chromosome repair in response to certain types of DNA damage, provide a means of investigating the increased chromosome fragility characteristic of Fanconi's anemia. By a recently developed technique using 33258 Hoechst and 5 bromodeoxyuridine, it was observed that the baseline frequency of sister chromatid exchanges in phytohemagglutinin stimulated lymphocytes from four males with Fanconi's anemia differed little from that of normal lymphocytes. However, addition of the bifunctional alkylating agent mitomycin C (0.01 or 0.03 μg/ml) to the Fanconi's anemia cells during culture induces less than half of the increase in exchanges found in identically treated normal lymphocytes. This reduced increment in exchanges is accompanied by a partial suppression of mitosis and a marked increase in chromatid breaks and rearrangements. Many of these events occur at sites of incomplete chromatid interchange. The increase in sister chromatid exchanges induced in Fanconi's anemia lymphocytes by the monofunctional alkylating agent ethylmethane sulfonate (0.25 mg/ml) was slightly less than that in normal cells. Lymphocytes from two sets of parents of the patients with Fanconi's anemia exhibited a normal response to alkylating agents, while dermal fibroblasts from two different parients with Fanconi's anemia reacted to mitomycin C with an increase in chromatid breaks, but a nearly normal increment of sister chromatid exchanges. The results suggest that chromocomal breaks and rearrangements in Fanconi's anemia lymphocytes may result from a defect in a form of repair of DNA damage.",
author = "Latt, {S. A.} and G. Stetten and Juergens, {L. A.} and Buchanan, {G. R.} and Gerald, {P. S.}",
year = "1975",
doi = "10.1073/pnas.72.10.4066",
language = "English (US)",
volume = "72",
pages = "4066--4070",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "10",

}

TY - JOUR

T1 - Induction by alkylating agents of sister chromatid exchanges and chromatid breaks in Fanconi's anemia

AU - Latt, S. A.

AU - Stetten, G.

AU - Juergens, L. A.

AU - Buchanan, G. R.

AU - Gerald, P. S.

PY - 1975

Y1 - 1975

N2 - Sister chromatid exchanges, which may reflect chromosome repair in response to certain types of DNA damage, provide a means of investigating the increased chromosome fragility characteristic of Fanconi's anemia. By a recently developed technique using 33258 Hoechst and 5 bromodeoxyuridine, it was observed that the baseline frequency of sister chromatid exchanges in phytohemagglutinin stimulated lymphocytes from four males with Fanconi's anemia differed little from that of normal lymphocytes. However, addition of the bifunctional alkylating agent mitomycin C (0.01 or 0.03 μg/ml) to the Fanconi's anemia cells during culture induces less than half of the increase in exchanges found in identically treated normal lymphocytes. This reduced increment in exchanges is accompanied by a partial suppression of mitosis and a marked increase in chromatid breaks and rearrangements. Many of these events occur at sites of incomplete chromatid interchange. The increase in sister chromatid exchanges induced in Fanconi's anemia lymphocytes by the monofunctional alkylating agent ethylmethane sulfonate (0.25 mg/ml) was slightly less than that in normal cells. Lymphocytes from two sets of parents of the patients with Fanconi's anemia exhibited a normal response to alkylating agents, while dermal fibroblasts from two different parients with Fanconi's anemia reacted to mitomycin C with an increase in chromatid breaks, but a nearly normal increment of sister chromatid exchanges. The results suggest that chromocomal breaks and rearrangements in Fanconi's anemia lymphocytes may result from a defect in a form of repair of DNA damage.

AB - Sister chromatid exchanges, which may reflect chromosome repair in response to certain types of DNA damage, provide a means of investigating the increased chromosome fragility characteristic of Fanconi's anemia. By a recently developed technique using 33258 Hoechst and 5 bromodeoxyuridine, it was observed that the baseline frequency of sister chromatid exchanges in phytohemagglutinin stimulated lymphocytes from four males with Fanconi's anemia differed little from that of normal lymphocytes. However, addition of the bifunctional alkylating agent mitomycin C (0.01 or 0.03 μg/ml) to the Fanconi's anemia cells during culture induces less than half of the increase in exchanges found in identically treated normal lymphocytes. This reduced increment in exchanges is accompanied by a partial suppression of mitosis and a marked increase in chromatid breaks and rearrangements. Many of these events occur at sites of incomplete chromatid interchange. The increase in sister chromatid exchanges induced in Fanconi's anemia lymphocytes by the monofunctional alkylating agent ethylmethane sulfonate (0.25 mg/ml) was slightly less than that in normal cells. Lymphocytes from two sets of parents of the patients with Fanconi's anemia exhibited a normal response to alkylating agents, while dermal fibroblasts from two different parients with Fanconi's anemia reacted to mitomycin C with an increase in chromatid breaks, but a nearly normal increment of sister chromatid exchanges. The results suggest that chromocomal breaks and rearrangements in Fanconi's anemia lymphocytes may result from a defect in a form of repair of DNA damage.

UR - http://www.scopus.com/inward/record.url?scp=0016720309&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016720309&partnerID=8YFLogxK

U2 - 10.1073/pnas.72.10.4066

DO - 10.1073/pnas.72.10.4066

M3 - Article

VL - 72

SP - 4066

EP - 4070

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 10

ER -