TY - JOUR
T1 - Induction of cyclic AMP-binding proteins by dibutyryl cyclic AMP in mouse neuroblastoma cells
AU - Prashad, Nagindra
AU - Rosenberg, Roger N.
N1 - Funding Information:
We acknowledge the excellent technical assistance of Ms. Betsy Wischmeyer. We thank Drs. R. Butow, M. Morrison, K. Nath and A.J. Bednarz for helpful comments. This research was supported by funds from N.I.H. Grant CA 17065, USPHS l-R01 CA 21602 and Leland Fikes Foundation, Inc.
PY - 1978/4/3
Y1 - 1978/4/3
N2 - Mouse neuroblastoma cells grown in the presence of 1 mM N6, O2′-dibutyryl-cyclic AMP showed a 3-fold increase in cyclic AMP-binding proteins. The role of dibutyryl cyclic AMP in the introduction of cyclic AMP-binding proteins in these cells has been studied. Induced cyclic AMP-binding proteins were observed in the cytoplasm 15 h after dibutyryl cyclic AMP treatment. The increase in cyclic AMP-binding proteins required RNA and protein synthesis. It is suggested that the 15-h lag occurs at the post-transcriptional and/or translational level. Cyclic AMP-binding proteins are found in both soluble and particulate cell fractions. Dibutyryl cyclic AMP increased binding proteins in both fractions. The control and dibutyryl cyclic AMP-induced binding proteins showed similar affinity for cyclic AMP. The data indicate that dibutyryl cyclic AMP caused the following sequential events: a 12-fold increase in cyclic AMP levels; a 40% increase in phosphodiesterase activity; and a 300% (3-fold) increase in cyclic AMP-binding proteins. It is suggested that the differentiation of mouse neuroblastoma cells involves increased levels of cyclic AMP and cyclic AMP-binding proteins.
AB - Mouse neuroblastoma cells grown in the presence of 1 mM N6, O2′-dibutyryl-cyclic AMP showed a 3-fold increase in cyclic AMP-binding proteins. The role of dibutyryl cyclic AMP in the introduction of cyclic AMP-binding proteins in these cells has been studied. Induced cyclic AMP-binding proteins were observed in the cytoplasm 15 h after dibutyryl cyclic AMP treatment. The increase in cyclic AMP-binding proteins required RNA and protein synthesis. It is suggested that the 15-h lag occurs at the post-transcriptional and/or translational level. Cyclic AMP-binding proteins are found in both soluble and particulate cell fractions. Dibutyryl cyclic AMP increased binding proteins in both fractions. The control and dibutyryl cyclic AMP-induced binding proteins showed similar affinity for cyclic AMP. The data indicate that dibutyryl cyclic AMP caused the following sequential events: a 12-fold increase in cyclic AMP levels; a 40% increase in phosphodiesterase activity; and a 300% (3-fold) increase in cyclic AMP-binding proteins. It is suggested that the differentiation of mouse neuroblastoma cells involves increased levels of cyclic AMP and cyclic AMP-binding proteins.
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U2 - 10.1016/0304-4165(78)90079-X
DO - 10.1016/0304-4165(78)90079-X
M3 - Article
C2 - 205271
AN - SCOPUS:0018179225
VL - 539
SP - 459
EP - 469
JO - Biochimica et Biophysica Acta - General Subjects
JF - Biochimica et Biophysica Acta - General Subjects
SN - 0304-4165
IS - 4
ER -