Inflammation, immune reactivity, and angiogenesis in a severe combined immunodeficiency model of rheumatoid arthritis

Laurie S. Davis, Marian Sackler, Ruth I. Brezinschek, Ellis Lightfoot, Jennifer L. Bailey, Nancy Oppenheimer-Marks, Peter E. Lipsky

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Severe combined immunodeficiency (SCID) mice were engrafted with rheumatoid arthritis (RA) synovium and evaluated to determine whether RA synovial morphology and function were maintained in the RA-SCID grafts. The four major components of RA synovitis, inflammation, immune reactivity, angiogenesis, and synovial hyperplasia persisted in RASCID grafts for 12 weeks. Retention of chronic inflammatory infiltrates was demonstrated by histological evaluation and by immunohistology for CD3, CD20, and CD68. Staining for CD68 also revealed that the grafts had undergone reorganization of the tissue, possibly as a result of fibroblast hyperplasia. Immune and inflammatory components were confirmed by the detection of human immunoglobulins and human interleukin-6 in serum samples obtained from grafted animals. Human blood vessels were detected by dense expression of CD31. Small vessels persistently expressed the vitronectin receptor, αvβ3, a marker of angiogenesis. All vessels expressed VAP-1, a marker of activated endothelial cells. Finally, the grafts retained the ability to support immigration by human leukocytes, as demonstrated by the functional capacity to recruit adoptively transferred 5- (and -6)-carboxyfluorescein diacetate succinimidyl ester-labeled T cells. T cells entering the RA-SCID grafts became activated and produced interferon-γ, as detected by reverse transcriptase-polymerase chain reaction analysis. These studies demonstrate that the RA-SCID model maintains many of the phenotypic and functional features of the inflamed RA synovium.

Original languageEnglish (US)
Pages (from-to)357-367
Number of pages11
JournalAmerican Journal of Pathology
Volume160
Issue number1
StatePublished - 2002

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Severe Combined Immunodeficiency
Rheumatoid Arthritis
Inflammation
Transplants
Synovial Membrane
Hyperplasia
Integrin alphaVbeta3
T-Lymphocytes
Synovitis
Emigration and Immigration
Reverse Transcriptase Polymerase Chain Reaction
Interferons
Blood Vessels
Immunoglobulins
Interleukin-6
Leukocytes
Endothelial Cells
Fibroblasts
Staining and Labeling
Serum

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Davis, L. S., Sackler, M., Brezinschek, R. I., Lightfoot, E., Bailey, J. L., Oppenheimer-Marks, N., & Lipsky, P. E. (2002). Inflammation, immune reactivity, and angiogenesis in a severe combined immunodeficiency model of rheumatoid arthritis. American Journal of Pathology, 160(1), 357-367.

Inflammation, immune reactivity, and angiogenesis in a severe combined immunodeficiency model of rheumatoid arthritis. / Davis, Laurie S.; Sackler, Marian; Brezinschek, Ruth I.; Lightfoot, Ellis; Bailey, Jennifer L.; Oppenheimer-Marks, Nancy; Lipsky, Peter E.

In: American Journal of Pathology, Vol. 160, No. 1, 2002, p. 357-367.

Research output: Contribution to journalArticle

Davis, LS, Sackler, M, Brezinschek, RI, Lightfoot, E, Bailey, JL, Oppenheimer-Marks, N & Lipsky, PE 2002, 'Inflammation, immune reactivity, and angiogenesis in a severe combined immunodeficiency model of rheumatoid arthritis', American Journal of Pathology, vol. 160, no. 1, pp. 357-367.
Davis LS, Sackler M, Brezinschek RI, Lightfoot E, Bailey JL, Oppenheimer-Marks N et al. Inflammation, immune reactivity, and angiogenesis in a severe combined immunodeficiency model of rheumatoid arthritis. American Journal of Pathology. 2002;160(1):357-367.
Davis, Laurie S. ; Sackler, Marian ; Brezinschek, Ruth I. ; Lightfoot, Ellis ; Bailey, Jennifer L. ; Oppenheimer-Marks, Nancy ; Lipsky, Peter E. / Inflammation, immune reactivity, and angiogenesis in a severe combined immunodeficiency model of rheumatoid arthritis. In: American Journal of Pathology. 2002 ; Vol. 160, No. 1. pp. 357-367.
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