Influence of IgH V-region genes on the growth kinetics of a murine B cell leukemia (BCL1)

R. Ciavarra, J. Forman

Research output: Contribution to journalArticle

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Abstract

The growth kinetics of an IgM-bearing B cell leukemia of BALB/c (Ig-Ia) origin, designated BCL1, has been investigated in 2 allotype immunoglobulin (Ig) heavy (H) chain congenic strains, C.B-20 (Ig-1b) and C.AL-20 (Ig-1(d)), and an (H) chain recombinant strain, BAB-14 (Ig-1a/1b), that carries Ig-1a genes in the variable (V)-region and Ig-1b genes in the constant (C)-region. When large numbers (106 to 107) of BCL1 cells were injected into these mice, leukemia, as measured by the appearance of leukemic cells in peripheral blood with subsequent mortality, did not occur in C.B-20, was delayed in C.AL-20, and progressed at the same rate in BAB-14 relative to BALB/c control mice. These results indicate that an immune response directed against an antigen encoded for by an H chain V region gene (idiotype or variable-region allotype) or linked gene (minor histocompatibility antigen) prevents the growth of the BCL1 leukemia in the C.B-20 mice. Tumor resistance appears to be due to T cell activity adoptive transfer of such cells from C.B.-20 tumor rejectors protected sublethally irradiated recipients from subsequent tumor challenge. Although H-2 restricted, anti-BCL1 cytotoxic cells were detected in C.B-20 mice challenged in vivo and restimulated in vitro with BCL1 cells, evidence is discussed that suggests that the resistance observed is not due to these effector cells. The resistance of allotype congenic mice to BCL1 was not absolute; a small inoculum (102) was as lethal in C.B-20 and C.AL-20 as BALB/c mice. Thus, Ig-encoded cell surface antigens, although immunogenic, in no way ensure ultimate host survival.

Original languageEnglish (US)
Pages (from-to)54-58
Number of pages5
JournalJournal of Immunology
Volume126
Issue number1
StatePublished - Jan 1 1981

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B-Cell Leukemia
Immunoglobulins
Growth
Immunoglobulin Genes
Genes
Leukemia
Minor Histocompatibility Antigens
Congenic Mice
Neoplasms
Immunoglobulin Heavy Chains
Adoptive Transfer
Surface Antigens
Immunoglobulin M
T-Lymphocytes
Mortality

ASJC Scopus subject areas

  • Immunology

Cite this

Influence of IgH V-region genes on the growth kinetics of a murine B cell leukemia (BCL1). / Ciavarra, R.; Forman, J.

In: Journal of Immunology, Vol. 126, No. 1, 01.01.1981, p. 54-58.

Research output: Contribution to journalArticle

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