Inhibition of Escherichia coli viability by external guide sequences complementary to two essential genes

Jeffrey McKinney, Cecilia Guerrier-Takada, Donna Wesolowski, Sidney Altman

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Narrow spectrum antimicrobial activity has been designed to reduce the expression of two essential genes, one coding for the protein subunit of RNase P (C5 protein) and one for gyrase (gyrase A). In both cases, external guide sequences (EGS) have been designed to complex with either mRNA. Using the EGS technology, the level of microbial viability is reduced to less than 10% of the wild-type strain. The EGSs are additive when used together and depend on the number of nucleotides paired when attacking gyrase A mRNA. In the case of gyrase A, three nucleotides unpaired out of a 15-mer EGS still favor complete inhibition by the EGS but five unpaired nucleotides do not.

Original languageEnglish (US)
Pages (from-to)6605-6610
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number12
DOIs
StatePublished - Jun 5 2001

Keywords

  • Rnase P
  • tRNA processing

ASJC Scopus subject areas

  • General

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