Inhibition of growth and cholesterol synthesis in breast cancer cells by oxidation products of β-carotene

Xiaoming Hu, Kevin M. White, Neil E. Jacobsen, David J. Mangelsdorf, Louise M. Canfield

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We have isolated and chemically characterized a polar oxidation product of β-carotene and tested the effect of a highly enriched fraction containing this compound on the growth and metabolism of breast cancer (MCF-7) cells. This fraction strongly inhibits cell growth and cholesterol synthesis in MCF-7 cells. Pretreatment of the cells with mevalonate overcomes inhibition of cell growth by the oxidized fraction. Addition of the antioxidant butylated hydroxytoluene protects against inhibition of the growth of MCF-7 cells by β-carotene but not by the oxidized fraction. Pretreatment of cells with mevalonate overcomes inhibition of cell growth by oxidation products of β-carotene but not by retinoic acid. The oxidized fraction neither stimulates activity nor inhibits binding of retinoic acid to its nuclear receptors (RXR-α, RXR-β, RXR-γ, RAR-α, RAR-β, RAR-γ, and peroxisome proliferation receptors) in transfection assays. Mevalonate does not protect retinoic acid-induced growth inhibition of MCF-7 cells. The major compound in the inhibitory fraction was identified by mass spectrometry and nuclear magnetic resonance spectroscopy as 5,8-endoperoxy-2,3-dihydro-β-apocarotene-13-one. Our data suggest that the β-carotene oxidation products we have isolated represent a class of compounds not previously described with potential antineoplastic activity. Copyright (C) 1998 Elsevier Science Inc.

Original languageEnglish (US)
Pages (from-to)567-574
Number of pages8
JournalJournal of Nutritional Biochemistry
Volume9
Issue number10
DOIs
StatePublished - Oct 1998

Fingerprint

Carotenoids
Mevalonic Acid
Cell growth
Tretinoin
Cholesterol
MCF-7 Cells
Cells
Breast Neoplasms
Oxidation
Growth
Butylated Hydroxytoluene
Cytoplasmic and Nuclear Receptors
Metabolism
Antineoplastic Agents
Nuclear magnetic resonance spectroscopy
Mass spectrometry
Assays
Peroxisomes
Antioxidants
Transfection

Keywords

  • β-carotene
  • Cancer
  • Cholesterol
  • MCF-7 cells
  • Mevalonate
  • Oxidation

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Inhibition of growth and cholesterol synthesis in breast cancer cells by oxidation products of β-carotene. / Hu, Xiaoming; White, Kevin M.; Jacobsen, Neil E.; Mangelsdorf, David J.; Canfield, Louise M.

In: Journal of Nutritional Biochemistry, Vol. 9, No. 10, 10.1998, p. 567-574.

Research output: Contribution to journalArticle

Hu, Xiaoming ; White, Kevin M. ; Jacobsen, Neil E. ; Mangelsdorf, David J. ; Canfield, Louise M. / Inhibition of growth and cholesterol synthesis in breast cancer cells by oxidation products of β-carotene. In: Journal of Nutritional Biochemistry. 1998 ; Vol. 9, No. 10. pp. 567-574.
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