Inhibitory effects of voluntary wheel exercise on apoptosis in splenic lymphocyte subsets of C57BL/6 mice

C. P. Reddy Avula, A. R. Muthukumar, K. Zaman, R. McCarter, G. Fernandes

Research output: Contribution to journalArticle

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Abstract

Two-month-old mice were placed in cages with (Ex) or without exercise running wheels with free access to the wheel 24 h/day for 10 mo. An equal amount of food for both groups was provided daily. Ex mice ran an average of 33.67 km/wk initially, and exercise decreased gradually with age. Ex mice had gained an average of 43.5% less body weight at the end of the experiment. Although serum lipid peroxides were not altered by exercise, superoxide dismutase and glutathione peroxidase activities in serum were significantly increased. Flow cytometric analysis of spleen cells revealed an increased percentage of CD8+ T cells and a decreased percentage of CD19+ B cells in Ex mice (P < 0.05). Exercise decreased apoptosis in total splenocytes and CD4+ cells incubated with medium alone or with H2O2, dexamethasone, tumor necrosis factor-α (TNF-α), or anti-CD3 monoclonal antibody (P < 0.05) and CD8+ cells with medium alone or with TNF-α (P < 0.05). Even though exercise did not alter the intracellular cytokines (TNF-α and interleukin-2) or Fas ligand, it did significantly lower interferon-γ in CD4+ and CD8+ cells (P < 0.05). In summary, voluntary wheel exercise appears to decrease H2O2-induced apoptosis in immune cells as well as decrease interferon-γ production.

Original languageEnglish (US)
Pages (from-to)2546-2552
Number of pages7
JournalJournal of Applied Physiology
Volume91
Issue number6
StatePublished - 2001

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Lymphocyte Subsets
Inbred C57BL Mouse
Apoptosis
Tumor Necrosis Factor-alpha
Interferons
Fas Ligand Protein
Lipid Peroxides
Glutathione Peroxidase
Serum
Running
Dexamethasone
Superoxide Dismutase
Interleukin-2
B-Lymphocytes
Spleen
Monoclonal Antibodies
Body Weight
Cytokines
T-Lymphocytes
Food

Keywords

  • Anti-CD3
  • Antioxidant enzymes
  • Dexamethasone
  • HO
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Inhibitory effects of voluntary wheel exercise on apoptosis in splenic lymphocyte subsets of C57BL/6 mice. / Reddy Avula, C. P.; Muthukumar, A. R.; Zaman, K.; McCarter, R.; Fernandes, G.

In: Journal of Applied Physiology, Vol. 91, No. 6, 2001, p. 2546-2552.

Research output: Contribution to journalArticle

Reddy Avula, C. P. ; Muthukumar, A. R. ; Zaman, K. ; McCarter, R. ; Fernandes, G. / Inhibitory effects of voluntary wheel exercise on apoptosis in splenic lymphocyte subsets of C57BL/6 mice. In: Journal of Applied Physiology. 2001 ; Vol. 91, No. 6. pp. 2546-2552.
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