Initiation of translation by cricket paralysis virus IRES requires its translocation in the ribosome

Israel S. Fernández, Xiao Chen Bai, Garib Murshudov, Sjors H W Scheres, V. Ramakrishnan

Research output: Contribution to journalArticle

127 Scopus citations

Abstract

The cricket paralysis virus internal ribosome entry site (CrPV-IRES) is a folded structure in a viral mRNA that allows initiation of translation in the absence of any host initiation factors. By using recent advances in single-particle electron cryomicroscopy, we have solved the structure of CrPV-IRES bound to the ribosome of the yeast Kluyveromyces lactis in both the canonical and rotated states at overall resolutions of 3.7 and 3.8 Å, respectively. In both states, the pseudoknot PKI of the CrPV-IRES mimics a tRNA/mRNA interaction in the decoding center of the A site of the 40S ribosomal subunit. The structure and accompanying factor-binding data show that CrPV-IRES binding mimics a pretranslocation rather than initiation state of the ribosome. Translocation of the IRES by elongation factor 2 (eEF2) is required to bring the first codon of the mRNA into the A site and to allow the start of translation.

Original languageEnglish (US)
Pages (from-to)823-831
Number of pages9
JournalCell
Volume157
Issue number4
DOIs
StatePublished - May 8 2014

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Initiation of translation by cricket paralysis virus IRES requires its translocation in the ribosome'. Together they form a unique fingerprint.

  • Cite this