Initiation of translation by cricket paralysis virus IRES requires its translocation in the ribosome

Israel S. Fernández, Xiao Chen Bai, Garib Murshudov, Sjors H W Scheres, V. Ramakrishnan

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

The cricket paralysis virus internal ribosome entry site (CrPV-IRES) is a folded structure in a viral mRNA that allows initiation of translation in the absence of any host initiation factors. By using recent advances in single-particle electron cryomicroscopy, we have solved the structure of CrPV-IRES bound to the ribosome of the yeast Kluyveromyces lactis in both the canonical and rotated states at overall resolutions of 3.7 and 3.8 Å, respectively. In both states, the pseudoknot PKI of the CrPV-IRES mimics a tRNA/mRNA interaction in the decoding center of the A site of the 40S ribosomal subunit. The structure and accompanying factor-binding data show that CrPV-IRES binding mimics a pretranslocation rather than initiation state of the ribosome. Translocation of the IRES by elongation factor 2 (eEF2) is required to bring the first codon of the mRNA into the A site and to allow the start of translation.

Original languageEnglish (US)
Pages (from-to)823-831
Number of pages9
JournalCell
Volume157
Issue number4
DOIs
StatePublished - May 8 2014

Fingerprint

Gryllidae
Ribosomes
Viruses
Paralysis
Messenger RNA
Eukaryotic Small Ribosome Subunits
Peptide Elongation Factor 2
Kluyveromyces
Cryoelectron Microscopy
Peptide Initiation Factors
Transfer RNA
Codon
Yeast
Decoding
Yeasts
Binding Sites
Internal Ribosome Entry Sites
Electrons

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Initiation of translation by cricket paralysis virus IRES requires its translocation in the ribosome. / Fernández, Israel S.; Bai, Xiao Chen; Murshudov, Garib; Scheres, Sjors H W; Ramakrishnan, V.

In: Cell, Vol. 157, No. 4, 08.05.2014, p. 823-831.

Research output: Contribution to journalArticle

Fernández, Israel S. ; Bai, Xiao Chen ; Murshudov, Garib ; Scheres, Sjors H W ; Ramakrishnan, V. / Initiation of translation by cricket paralysis virus IRES requires its translocation in the ribosome. In: Cell. 2014 ; Vol. 157, No. 4. pp. 823-831.
@article{54ee6344a4804ab6b6b23880b95c74a4,
title = "Initiation of translation by cricket paralysis virus IRES requires its translocation in the ribosome",
abstract = "The cricket paralysis virus internal ribosome entry site (CrPV-IRES) is a folded structure in a viral mRNA that allows initiation of translation in the absence of any host initiation factors. By using recent advances in single-particle electron cryomicroscopy, we have solved the structure of CrPV-IRES bound to the ribosome of the yeast Kluyveromyces lactis in both the canonical and rotated states at overall resolutions of 3.7 and 3.8 {\AA}, respectively. In both states, the pseudoknot PKI of the CrPV-IRES mimics a tRNA/mRNA interaction in the decoding center of the A site of the 40S ribosomal subunit. The structure and accompanying factor-binding data show that CrPV-IRES binding mimics a pretranslocation rather than initiation state of the ribosome. Translocation of the IRES by elongation factor 2 (eEF2) is required to bring the first codon of the mRNA into the A site and to allow the start of translation.",
author = "Fern{\'a}ndez, {Israel S.} and Bai, {Xiao Chen} and Garib Murshudov and Scheres, {Sjors H W} and V. Ramakrishnan",
year = "2014",
month = "5",
day = "8",
doi = "10.1016/j.cell.2014.04.015",
language = "English (US)",
volume = "157",
pages = "823--831",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "4",

}

TY - JOUR

T1 - Initiation of translation by cricket paralysis virus IRES requires its translocation in the ribosome

AU - Fernández, Israel S.

AU - Bai, Xiao Chen

AU - Murshudov, Garib

AU - Scheres, Sjors H W

AU - Ramakrishnan, V.

PY - 2014/5/8

Y1 - 2014/5/8

N2 - The cricket paralysis virus internal ribosome entry site (CrPV-IRES) is a folded structure in a viral mRNA that allows initiation of translation in the absence of any host initiation factors. By using recent advances in single-particle electron cryomicroscopy, we have solved the structure of CrPV-IRES bound to the ribosome of the yeast Kluyveromyces lactis in both the canonical and rotated states at overall resolutions of 3.7 and 3.8 Å, respectively. In both states, the pseudoknot PKI of the CrPV-IRES mimics a tRNA/mRNA interaction in the decoding center of the A site of the 40S ribosomal subunit. The structure and accompanying factor-binding data show that CrPV-IRES binding mimics a pretranslocation rather than initiation state of the ribosome. Translocation of the IRES by elongation factor 2 (eEF2) is required to bring the first codon of the mRNA into the A site and to allow the start of translation.

AB - The cricket paralysis virus internal ribosome entry site (CrPV-IRES) is a folded structure in a viral mRNA that allows initiation of translation in the absence of any host initiation factors. By using recent advances in single-particle electron cryomicroscopy, we have solved the structure of CrPV-IRES bound to the ribosome of the yeast Kluyveromyces lactis in both the canonical and rotated states at overall resolutions of 3.7 and 3.8 Å, respectively. In both states, the pseudoknot PKI of the CrPV-IRES mimics a tRNA/mRNA interaction in the decoding center of the A site of the 40S ribosomal subunit. The structure and accompanying factor-binding data show that CrPV-IRES binding mimics a pretranslocation rather than initiation state of the ribosome. Translocation of the IRES by elongation factor 2 (eEF2) is required to bring the first codon of the mRNA into the A site and to allow the start of translation.

UR - http://www.scopus.com/inward/record.url?scp=84900435661&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84900435661&partnerID=8YFLogxK

U2 - 10.1016/j.cell.2014.04.015

DO - 10.1016/j.cell.2014.04.015

M3 - Article

VL - 157

SP - 823

EP - 831

JO - Cell

JF - Cell

SN - 0092-8674

IS - 4

ER -