Insulin enhances glucose-stimulated insulin secretion in healthy humans

Clara Bouche, Ximena Lopez, Amy Fleischman, Aaron M. Cypess, Sheila O'Shea, Darko Stefanovski, Richard N. Bergman, Eduard Rogatsky, Daniel T. Stein, C. Ronald Kahn, Rohit N. Kulkarni, Allison B. Goldfine

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Islet β-cells express both insulin receptors and insulin-signaling proteins. Recent evidence from rodents in vivo and from islets isolated from rodents or humans suggests that the insulin signaling pathway is physiologically important for glucose sensing. We evaluated whether insulin regulates β-cell function in healthy humans in vivo. Glucose-induced insulin secretion was assessed in healthy humans following 4-h saline (low insulin/sham clamp) or isoglycemic-hyperinsulinemic (high insulin) clamps using B28-Asp insulin that could be immunologically distinguished from endogenous insulin. Insulin and C-peptide clearance were evaluated to understand the impact of hyperinsulinemia on estimates of β-cell function. Preexposure to exogenous insulin increased the endogenous insulin secretory response to glucose by ≈40%. C-peptide response also increased, although not to the level predicted by insulin. Insulin clearance was not saturated at hyperinsulinemia, but metabolic clearance of C-peptide, assessed by infusion of stable isotope-labeled C-peptide, increased modestly during hyperinsulinemic clamp. These studies demonstrate that insulin potentiates glucose-stimulated insulin secretion in vivo in healthy humans. In addition, hyperinsulinemia increases C-peptide clearance, which may lead to modest underestimation of β-cell secretory response when using these methods during prolonged dynamic testing.

Original languageEnglish (US)
Pages (from-to)4770-4775
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number10
DOIs
StatePublished - Mar 9 2010

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Insulin
Glucose
C-Peptide
Hyperinsulinism
Rodentia
Insulin Aspart
Insulin Receptor
Islets of Langerhans
Isotopes

Keywords

  • Beta cell
  • C-peptide
  • Insulin clearance
  • Insulin resistance
  • Type 2 diabetes mellitus

ASJC Scopus subject areas

  • General

Cite this

Insulin enhances glucose-stimulated insulin secretion in healthy humans. / Bouche, Clara; Lopez, Ximena; Fleischman, Amy; Cypess, Aaron M.; O'Shea, Sheila; Stefanovski, Darko; Bergman, Richard N.; Rogatsky, Eduard; Stein, Daniel T.; Kahn, C. Ronald; Kulkarni, Rohit N.; Goldfine, Allison B.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, No. 10, 09.03.2010, p. 4770-4775.

Research output: Contribution to journalArticle

Bouche, C, Lopez, X, Fleischman, A, Cypess, AM, O'Shea, S, Stefanovski, D, Bergman, RN, Rogatsky, E, Stein, DT, Kahn, CR, Kulkarni, RN & Goldfine, AB 2010, 'Insulin enhances glucose-stimulated insulin secretion in healthy humans', Proceedings of the National Academy of Sciences of the United States of America, vol. 107, no. 10, pp. 4770-4775. https://doi.org/10.1073/pnas.1000002107
Bouche, Clara ; Lopez, Ximena ; Fleischman, Amy ; Cypess, Aaron M. ; O'Shea, Sheila ; Stefanovski, Darko ; Bergman, Richard N. ; Rogatsky, Eduard ; Stein, Daniel T. ; Kahn, C. Ronald ; Kulkarni, Rohit N. ; Goldfine, Allison B. / Insulin enhances glucose-stimulated insulin secretion in healthy humans. In: Proceedings of the National Academy of Sciences of the United States of America. 2010 ; Vol. 107, No. 10. pp. 4770-4775.
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