Interaction of the human papillomavirus type 16 E6 oncoprotein with wild-type and mutant human p53 proteins

Martin Scheffner, Takashi Takahashi, Jon M. Huibregtse, John D. Minna, Peter M. Howley

Research output: Contribution to journalArticle

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Abstract

The E6 oncoproteins encoded by the cancer-associated human papillomaviruses (HPVs) can associate with and promote the degradation of wild-type p53 in vitro. To gain further insight into this process, the ability of HPV-16 E6 to complex with and promote the degradation of mutant forms of p53 was studied. A correlation between binding and the targeted degradation of p53 was established. Mutant p53 proteins that bound HPV-16 E6 were targeted for degradation, whereas those that did not complex HPV-16 E6 were not degraded. Since the HPV-16 E6-promoted degradation involves the ubiquitin-dependent proteolysis pathway, specific mutations were made in the amino terminus of p53 to examine whether the E6 targeted degradation involved the N-end rule pathway. No requirement for destabilizing amino acids at the N terminus of p53 was found, nor was evidence found that HPV-16 E6 could provide this determinant in trans, indicating that the N-terminal rule pathway is not involved in the E6-promoted degradation of p53.

Original languageEnglish (US)
Pages (from-to)5100-5105
Number of pages6
JournalJournal of Virology
Volume66
Issue number8
StatePublished - Aug 1992

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Human papillomavirus 16
Papillomaviridae
Oncogene Proteins
mutants
degradation
Proteins
proteins
Mutant Proteins
Ubiquitin
Proteolysis
amino acid requirements
ubiquitin
oncogene proteins
proteolysis
Amino Acids
Mutation
mutation
neoplasms
Neoplasms

ASJC Scopus subject areas

  • Immunology

Cite this

Scheffner, M., Takahashi, T., Huibregtse, J. M., Minna, J. D., & Howley, P. M. (1992). Interaction of the human papillomavirus type 16 E6 oncoprotein with wild-type and mutant human p53 proteins. Journal of Virology, 66(8), 5100-5105.

Interaction of the human papillomavirus type 16 E6 oncoprotein with wild-type and mutant human p53 proteins. / Scheffner, Martin; Takahashi, Takashi; Huibregtse, Jon M.; Minna, John D.; Howley, Peter M.

In: Journal of Virology, Vol. 66, No. 8, 08.1992, p. 5100-5105.

Research output: Contribution to journalArticle

Scheffner, M, Takahashi, T, Huibregtse, JM, Minna, JD & Howley, PM 1992, 'Interaction of the human papillomavirus type 16 E6 oncoprotein with wild-type and mutant human p53 proteins', Journal of Virology, vol. 66, no. 8, pp. 5100-5105.
Scheffner, Martin ; Takahashi, Takashi ; Huibregtse, Jon M. ; Minna, John D. ; Howley, Peter M. / Interaction of the human papillomavirus type 16 E6 oncoprotein with wild-type and mutant human p53 proteins. In: Journal of Virology. 1992 ; Vol. 66, No. 8. pp. 5100-5105.
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