Interactions of cellular proteins involved in the transcriptional regulation of the human immunodeficiency virus.

J. A. Garcia, F. K. Wu, R. Mitsuyasu, R. B. Gaynor

Research output: Contribution to journalArticle

158 Citations (Scopus)

Abstract

The human immunodeficiency virus (HIV) is a human retrovirus which is the etiologic agent of the acquired immunodeficiency syndrome. To study the cellular factors involved in the transcriptional regulation of this virus, we performed DNase I footprinting of the viral LTR using partially purified HeLa cell extracts. Five regions of the viral LTR appear critical for DNA binding of cellular proteins. These include the negative regulatory, enhancer, SP1, TATA and untranslated regions. Deletion mutagenesis of these binding domains has significant effects on the basal level of transcription and the ability to be induced by the viral tat protein. Mutations of either the negative regulatory or untranslated regions affect factor binding to the enhancer region. In addition, oligonucleotides complementary to several of the binding domains specifically compete for factor binding. These results suggest that interactions between several distinct cellular proteins are required for HIV transcriptional regulation.

Original languageEnglish (US)
Pages (from-to)3761-3770
Number of pages10
JournalEMBO Journal
Volume6
Issue number12
StatePublished - Dec 1 1987

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Untranslated Regions
Viruses
HIV
tat Gene Products
Human Immunodeficiency Virus Proteins
Nucleic Acid Regulatory Sequences
Deoxyribonuclease I
DNA-Binding Proteins
Viral Proteins
Retroviridae
Cell Extracts
HeLa Cells
Oligonucleotides
Mutagenesis
Acquired Immunodeficiency Syndrome
Proteins
Transcription
Mutation
DNA

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Garcia, J. A., Wu, F. K., Mitsuyasu, R., & Gaynor, R. B. (1987). Interactions of cellular proteins involved in the transcriptional regulation of the human immunodeficiency virus. EMBO Journal, 6(12), 3761-3770.

Interactions of cellular proteins involved in the transcriptional regulation of the human immunodeficiency virus. / Garcia, J. A.; Wu, F. K.; Mitsuyasu, R.; Gaynor, R. B.

In: EMBO Journal, Vol. 6, No. 12, 01.12.1987, p. 3761-3770.

Research output: Contribution to journalArticle

Garcia, JA, Wu, FK, Mitsuyasu, R & Gaynor, RB 1987, 'Interactions of cellular proteins involved in the transcriptional regulation of the human immunodeficiency virus.', EMBO Journal, vol. 6, no. 12, pp. 3761-3770.
Garcia, J. A. ; Wu, F. K. ; Mitsuyasu, R. ; Gaynor, R. B. / Interactions of cellular proteins involved in the transcriptional regulation of the human immunodeficiency virus. In: EMBO Journal. 1987 ; Vol. 6, No. 12. pp. 3761-3770.
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