Interferon signature gene expression is correlated with autoantibody profiles in patients with incomplete lupus syndromes

Q. Z. Li, J. Zhou, Y. Lian, B. Zhang, V. K. Branch, F. Carr-Johnson, D. R. Karp, C. Mohan, E. K. Wakeland, N. J. Olsen

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Interferon (IFN) signature genes have been shown to be expressed highly in peripheral blood of patients with systemic lupus erythematosus (SLE), especially in the presence of active disease. However, the expression of this gene signature in individuals with incomplete forms of lupus and the pathogenic relationship between IFN signature genes and autoantibody production have not been explored fully. In the present study, we examined the gene expression and autoantibody profiles of patients diagnosed with incomplete lupus erythematosus (ILE) to determine correlations of the gene expression signature with autoantibody production. Gene expression analysis was carried out on the 24K Illumina Human Refseq-8 arrays using blood samples from 84 subjects, including patients with SLE (n = 27) or ILE (n = 24), first-degree relatives (FDR) of these patients (n = 22) and non-autoimmune control (NC) individuals (n = 11). Autoantibody expression was measured using standard immunoassays and autoantigen proteomic arrays. Up-regulation of a set of 63 IFN signature genes was seen in 83% of SLE patients and 50% of ILE patients. High levels of IFN gene expression in ILE and SLE showed significant correlations with the expression of a subset of IgG autoantibodies, including chromatin, dsDNA, dsRNA, U1snRNP, Ro/SSA, La/SSB, topoisomerase I and Scl 70, while low IFN levels were correlated with immunoglobulin (Ig)M autoreactivity. These studies suggest that in patients with ILE the IFN gene expression signature may identify a subset of these individuals who are at risk for disease progression. Furthermore, high levels of alpha IFN may promote autoantibody class-switch from IgM to the more pathogenic IgG class.

Original languageEnglish (US)
Pages (from-to)281-291
Number of pages11
JournalClinical and Experimental Immunology
Volume159
Issue number3
DOIs
StatePublished - Mar 2010

Fingerprint

Transcriptome
Autoantibodies
Interferons
Systemic Lupus Erythematosus
Immunoglobulin M
Immunoglobulin G
Genes
Gene Expression
Type I DNA Topoisomerase
Autoantigens
Immunoassay
Interferon-alpha
Proteomics
Chromatin
Disease Progression
Up-Regulation

Keywords

  • Autoantibodies
  • Autoimmune disease
  • Gene expression profiling
  • IFN-α
  • SLE

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Interferon signature gene expression is correlated with autoantibody profiles in patients with incomplete lupus syndromes. / Li, Q. Z.; Zhou, J.; Lian, Y.; Zhang, B.; Branch, V. K.; Carr-Johnson, F.; Karp, D. R.; Mohan, C.; Wakeland, E. K.; Olsen, N. J.

In: Clinical and Experimental Immunology, Vol. 159, No. 3, 03.2010, p. 281-291.

Research output: Contribution to journalArticle

Li, Q. Z. ; Zhou, J. ; Lian, Y. ; Zhang, B. ; Branch, V. K. ; Carr-Johnson, F. ; Karp, D. R. ; Mohan, C. ; Wakeland, E. K. ; Olsen, N. J. / Interferon signature gene expression is correlated with autoantibody profiles in patients with incomplete lupus syndromes. In: Clinical and Experimental Immunology. 2010 ; Vol. 159, No. 3. pp. 281-291.
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