Interleukin-1B

A clinically relevant urinary marker

S. M. Martins, D. J. Darlin, P. M. Lad, P. E. Zimmern

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Urinary cytokines as markers for the intravesical inflammatory response have become an active area of research. Interleukin-1b, a well studied and early produced cytokine in the immune recognition cascade, was evaluated. After extensive analysis of 56 control and study group urine samples, a simplified and reliable protocol for the preparation of urine before cytokine analysis was devised. The application of an available serum interleukin-1b enzyme-linked immunosorbent assay kit for urinary interleukin-1b analysis was then tested. Finally, a reference value for interleukin-1b in normal human urine was established and urinary interleukin-1b was measured in various bladder diseases. The normal and interstitial cystitis groups showed no interleukin-1b elevation. Significant elevation of urinary interleukin-1b was found in patients with bacterial cystitis compared to the interstitial cystitis and control groups (p <0.001). Of the patients with bladder tumors 58% showed elevation of urinary interleukin-1b (p <0.001). Urinary interleukin-1b may be used as a marker to distinguish between bacterial and interstitial cystitis. The absence of urinary interleukin-1b in interstitial cystitis argues against an immunological or autoimmune etiology of the disorder. This study represents an important methodological approach to cytokine subtyping of bladder diseases.

Original languageEnglish (US)
Pages (from-to)1198-1201
Number of pages4
JournalJournal of Urology
Volume151
Issue number5
StatePublished - 1994

Fingerprint

Interleukins
Interstitial Cystitis
Urinary Bladder Diseases
Cytokines
Urine
Control Groups
Cystitis
Urinary Bladder Neoplasms
Reference Values
Enzyme-Linked Immunosorbent Assay

Keywords

  • cystitis
  • cytokines
  • interleukin-1b
  • urine

ASJC Scopus subject areas

  • Urology

Cite this

Martins, S. M., Darlin, D. J., Lad, P. M., & Zimmern, P. E. (1994). Interleukin-1B: A clinically relevant urinary marker. Journal of Urology, 151(5), 1198-1201.

Interleukin-1B : A clinically relevant urinary marker. / Martins, S. M.; Darlin, D. J.; Lad, P. M.; Zimmern, P. E.

In: Journal of Urology, Vol. 151, No. 5, 1994, p. 1198-1201.

Research output: Contribution to journalArticle

Martins, SM, Darlin, DJ, Lad, PM & Zimmern, PE 1994, 'Interleukin-1B: A clinically relevant urinary marker', Journal of Urology, vol. 151, no. 5, pp. 1198-1201.
Martins SM, Darlin DJ, Lad PM, Zimmern PE. Interleukin-1B: A clinically relevant urinary marker. Journal of Urology. 1994;151(5):1198-1201.
Martins, S. M. ; Darlin, D. J. ; Lad, P. M. ; Zimmern, P. E. / Interleukin-1B : A clinically relevant urinary marker. In: Journal of Urology. 1994 ; Vol. 151, No. 5. pp. 1198-1201.
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