Interleukin-2 mediated activation of jak3 in the absence of a detectable 04 kda common 7 chain

T. S. Fenske, N. L. Farner, J. J. O'Shea, P. M. Sondel

Research output: Contribution to journalArticle

Abstract

The common 7 chain is a component of the interîeukin (IL)-2, IL-4, IL-7, IL-9, and IL-15 receptors. The central role of the common 7 chain in cytokine signaling is reflected by the fact that patients with mutations in the common 7 gene are affected with X-linked severe combined immune deficiency (SCID). One important mechanism by which the common 7 chain activates cytokine signal transduction is via the associated kinase JAK3. Recently, it has been demonstrated that mutations in the JAK3 gene can lead to autosomal SCID, a disease that phenotypically mimics X-linked SCID. This result underscores the importance of JAK3 in immune cell activation. In this study, we evaluate IL-2 responses by the Tf-1/3 cell line, an IL-2 receptor /?-expressing derivative of the myelomonocytic cell line Tf-1. Cellular proliferation occurs in an IL-2 dependent manner. However, a 64 IcDa common 7 chain was not detected in these cells, using either surface iodination or western blotting techniques which detect a 64 kDa common 7 chain in other cell types. Nevertheless, JAK3 phosphorylation by Tf-1/9 cells is induced by IL-2. Our results suggest the presence of a functional common 7 chain isoform of a different molecular weight in Tf-1/3 cells, but would also be consistent with the existence of a separate JAK3 activator induced in Tf-1/3 by IL-2.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number6
StatePublished - 1996

Fingerprint

interleukin-2
Interleukin-2
Severe Combined Immunodeficiency
Chemical activation
immunosuppression
Interleukin-15 Receptors
Genes
cells
Cells
Interleukin-9
Cytokines
interleukin-9
cytokines
Cell Line
Interleukin-7
Signal transduction
Mutation
cell lines
Phosphorylation
iodination

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Interleukin-2 mediated activation of jak3 in the absence of a detectable 04 kda common 7 chain. / Fenske, T. S.; Farner, N. L.; O'Shea, J. J.; Sondel, P. M.

In: FASEB Journal, Vol. 10, No. 6, 1996.

Research output: Contribution to journalArticle

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AU - Farner, N. L.

AU - O'Shea, J. J.

AU - Sondel, P. M.

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