Intestinal epithelial cells down-regulate macrophage tumor necrosis factor-alpha secretion: A mechanism for immune homeostasis in the gut-associated lymphoid tissue

Avery B. Nathens, Ori D. Rotstein, Alan P B Dackiw, John C. Marshall

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Abstract

Background. The gut lumen contains more than 106 organisms per gram of luminal contents. The mechanisms that limit the response of macrophages in the lamina propria to these microbial antigens are unknown, although an intrinsic defect in this mechanism may contribute to the development of inflammatory bowel disease. Intestinal epithelial cells (IEC) may play an important role in mediating tonic down-regulation of local immune cell activation. The purpose of this study was to discern whether IEC might modulate macrophage activation in response to a variety of microbial stimuli. Methods. Thioglycollate-elicited murine peritoneal macrophages were activated by endotoxin, zymosan, Escherichia coli, and Candida albicans in the presence or absence of IEC from the rat intestinal epithelial cell line IEC-6. Macrophage tumor necrosis factor-α (TNF-α) secretion was determined by enzyme-linked immunosorbent assay. Results. Lipopolysaccharide or zymosan-activated macrophages in coculture with IEC secreted significantly less TNF-α than macrophages cultured alone. The inhibitory effect of the IEC was dependent on their activation by lipopolysaccharide. Interleukin-1α production was not affected. IEC-mediated suppression of macrophage TNF-α secretion was reversed by indomethacin but not by neutralizing antibody to TGF-β. Conclusions. Lipopolysaccharide-activated IEC down-regulate macrophage TNF-α secretion in response to microbial stimuli through a prostanoid-mediated mechanism. IEC may mediate tonic down-regulation of immune cell activation in the gut-associated lymphoid tissue and may thereby regulate local and systemic inflammatory responses.

Original languageEnglish (US)
Pages (from-to)343-351
Number of pages9
JournalSurgery
Volume118
Issue number2
DOIs
StatePublished - 1995

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Lymphoid Tissue
Homeostasis
Down-Regulation
Tumor Necrosis Factor-alpha
Epithelial Cells
Macrophages
Lipopolysaccharides
Zymosan
Thioglycolates
Macrophage Activation
Peritoneal Macrophages
Coculture Techniques
Neutralizing Antibodies
Candida albicans
Interleukin-1
Inflammatory Bowel Diseases
Indomethacin
Prostaglandins
Mucous Membrane
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Surgery

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Intestinal epithelial cells down-regulate macrophage tumor necrosis factor-alpha secretion : A mechanism for immune homeostasis in the gut-associated lymphoid tissue. / Nathens, Avery B.; Rotstein, Ori D.; Dackiw, Alan P B; Marshall, John C.

In: Surgery, Vol. 118, No. 2, 1995, p. 343-351.

Research output: Contribution to journalArticle

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abstract = "Background. The gut lumen contains more than 106 organisms per gram of luminal contents. The mechanisms that limit the response of macrophages in the lamina propria to these microbial antigens are unknown, although an intrinsic defect in this mechanism may contribute to the development of inflammatory bowel disease. Intestinal epithelial cells (IEC) may play an important role in mediating tonic down-regulation of local immune cell activation. The purpose of this study was to discern whether IEC might modulate macrophage activation in response to a variety of microbial stimuli. Methods. Thioglycollate-elicited murine peritoneal macrophages were activated by endotoxin, zymosan, Escherichia coli, and Candida albicans in the presence or absence of IEC from the rat intestinal epithelial cell line IEC-6. Macrophage tumor necrosis factor-α (TNF-α) secretion was determined by enzyme-linked immunosorbent assay. Results. Lipopolysaccharide or zymosan-activated macrophages in coculture with IEC secreted significantly less TNF-α than macrophages cultured alone. The inhibitory effect of the IEC was dependent on their activation by lipopolysaccharide. Interleukin-1α production was not affected. IEC-mediated suppression of macrophage TNF-α secretion was reversed by indomethacin but not by neutralizing antibody to TGF-β. Conclusions. Lipopolysaccharide-activated IEC down-regulate macrophage TNF-α secretion in response to microbial stimuli through a prostanoid-mediated mechanism. IEC may mediate tonic down-regulation of immune cell activation in the gut-associated lymphoid tissue and may thereby regulate local and systemic inflammatory responses.",
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