Intestinal trefoil factor induces decay-accelerating factor expression and enhances the protective activities against complement activation in intestinal epithelial cells

A. Andoh, K. Kinoshita, I. Rosenberg, D. K. Podolsky

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Mucosal damage induces a massive influx of serum complement components into the lumen. The epithelium produces a number of factors that can potentially ameliorate injury including intestinal trefoil factor (ITF), a small protease-resistant peptide produced and secreted onto the mucosal surface by goblet cells, and decay-accelerating factor (DAF), a protein produced by columnar epithelium which protects the host tissue from autologous complement injury. However, coordination of these intrinsic defensive products has not been delineated. DAF protein and mRNA expression were evaluated by immunoblotting and Northern blotting, respectively. NF-κB-DNA binding activity and DAF promoter activity were assessed by an electrophoretic gel mobility shift assay and a reporter gene luciferase assay, respectively. ITF induced a dose- and time-dependent increase in DAF protein and mRNA expression in human (HT-29 and T84) and rat (IEC-6) intestinal epithelial cells. In differentiated T84 cells grown on cell culture inserts, basolateral stimulation with ITF strongly enhanced DAF expression, but apical stimulation had no effects. The C3 deposition induced by complement activation was significantly blocked by the treatment with ITF. In HT-29 cells, ITF increased the stability of DAF mRNA. ITF also enhanced the promoter activity of the DAF gene via NF-κB motif and induced activation of NF-κB-DNA binding activity. ITF promotes protection of epithelial cells from complement activation via up-regulation of DAF expression, contributing to a robust mucosal defense.

Original languageEnglish (US)
Pages (from-to)3887-3893
Number of pages7
JournalJournal of Immunology
Volume167
Issue number7
StatePublished - Oct 1 2001

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CD55 Antigens
Complement Activation
Epithelial Cells
B-Form DNA
Messenger RNA
Epithelium
HT29 Cells
Proteins
Goblet Cells
Trefoil Factor-3
Wounds and Injuries
Electrophoretic Mobility Shift Assay
Luciferases
Reporter Genes
Immunoblotting
Northern Blotting
Peptide Hydrolases
Up-Regulation
Cell Culture Techniques
Gels

ASJC Scopus subject areas

  • Immunology

Cite this

Intestinal trefoil factor induces decay-accelerating factor expression and enhances the protective activities against complement activation in intestinal epithelial cells. / Andoh, A.; Kinoshita, K.; Rosenberg, I.; Podolsky, D. K.

In: Journal of Immunology, Vol. 167, No. 7, 01.10.2001, p. 3887-3893.

Research output: Contribution to journalArticle

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