TY - JOUR
T1 - Is the sanctuary where Helicobacter pylori avoids antibacterial treatment intracellular?
AU - Engstrand, L.
AU - Graham, Dy
AU - Scheynius, A.
AU - Genta, R. M.
AU - El-Zaatari, Fak
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1997/11
Y1 - 1997/11
N2 - The sanctuary site where Helicobacter pylori evades antimicrobial therapy is unknown, but considerable data exist about an intracellular location for H pylori. Ten H pylori-infected volunteers received standard triple antimicrobial therapy for 2 weeks. Gastric mucosal biopsy specimens were obtained with jumbo forceps on therapy days 0, 3, 14, and 42. Hematoxylin-eosin staining was used for classification of gastritis and the Genta stain for the visualization of H pylori. Immunohistochemical staining was used to detect HLA-DR antigens, human heat shock protein (HSP60), and the bacterial HSP60 antigen. Bacterial HSP60 was expressed on the mucosal surface and within epithelial cells. No such expression of human HSP60 was found, which supports a bacterial origin for the intracellular HSP60. Coexpression of bacterial HSP60 and HLA-DR was always observed, indicating an ongoing local immune response. Infection was cleared on day 14, but when examined 4 weeks after completion of therapy, Genta staining indicated that only five volunteers remained free of H pylori. However, results of immunohistochemical staining were negative at this time for only two volunteers. Disappearance of intracellular expression of bacterial HSP60 remained after therapy and correlated with the intensity of chronic inflammatory cell infiltration. These data are consistent with the intracellular localization of H pylori having a role in inflammation and as a protective strategy against extracellular antibacterial activity.
AB - The sanctuary site where Helicobacter pylori evades antimicrobial therapy is unknown, but considerable data exist about an intracellular location for H pylori. Ten H pylori-infected volunteers received standard triple antimicrobial therapy for 2 weeks. Gastric mucosal biopsy specimens were obtained with jumbo forceps on therapy days 0, 3, 14, and 42. Hematoxylin-eosin staining was used for classification of gastritis and the Genta stain for the visualization of H pylori. Immunohistochemical staining was used to detect HLA-DR antigens, human heat shock protein (HSP60), and the bacterial HSP60 antigen. Bacterial HSP60 was expressed on the mucosal surface and within epithelial cells. No such expression of human HSP60 was found, which supports a bacterial origin for the intracellular HSP60. Coexpression of bacterial HSP60 and HLA-DR was always observed, indicating an ongoing local immune response. Infection was cleared on day 14, but when examined 4 weeks after completion of therapy, Genta staining indicated that only five volunteers remained free of H pylori. However, results of immunohistochemical staining were negative at this time for only two volunteers. Disappearance of intracellular expression of bacterial HSP60 remained after therapy and correlated with the intensity of chronic inflammatory cell infiltration. These data are consistent with the intracellular localization of H pylori having a role in inflammation and as a protective strategy against extracellular antibacterial activity.
KW - Antimicrobial therapy
KW - Carcinogen
KW - Gastritis
KW - Helicobacter pylori
KW - Intracellular compartment
UR - http://www.scopus.com/inward/record.url?scp=0030671053&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030671053&partnerID=8YFLogxK
U2 - 10.1093/ajcp/108.5.504
DO - 10.1093/ajcp/108.5.504
M3 - Article
C2 - 9353088
AN - SCOPUS:0030671053
SN - 0002-9173
VL - 108
SP - 504
EP - 509
JO - American journal of clinical pathology
JF - American journal of clinical pathology
IS - 5
ER -