Isolation and immortalization of patient-derived cell lines from muscle biopsy for disease modeling

Jerome D. Robin, Woody E. Wright, Yaqun Zou, Stacy C. Cossette, Michael W. Lawlor, Emanuela Gussoni

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

The generation of patient-specific cell lines represents an invaluable tool for diagnostic or translational research, and these cells can be collected from skin or muscle biopsy tissue available during the patient’s diagnostic workup. In this protocol, we describe a technique for live cell isolation from small amounts of muscle or skin tissue for primary cell culture. Additionally, we provide a technique for the immortalization of myogenic cell lines and fibroblast cell lines from primary cells. Once cell lines are immortalized, substantial expansion of patient-derived cells can be achieved. Immortalized cells are amenable to many downstream applications, including drug screening and in vitro correction of the genetic mutation. Altogether, these protocols provide a reliable tool to generate and preserve patient-derived cells for downstream applications.

Original languageEnglish (US)
Article numbere52307
JournalJournal of Visualized Experiments
Issue number95
DOIs
StatePublished - Jan 18 2015

Keywords

  • Biopsy
  • Cell freezing
  • Issue 95
  • Medicine
  • Myoblast immortalization
  • Myoblast purification
  • Skeletal muscle
  • Skin
  • Tissue dissociation

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Fingerprint Dive into the research topics of 'Isolation and immortalization of patient-derived cell lines from muscle biopsy for disease modeling'. Together they form a unique fingerprint.

Cite this