TY - JOUR
T1 - Isoproterenol-induced cardiomyopathy
T2 - Changes in cardiac enzymes and protection by methylprednisolone
AU - Mueller, E. A.
AU - Griffin, W. S T
AU - Wildenthal, K.
N1 - Funding Information:
This work was supported by grants from the National Heart and Lung Institute (HL 18087, HL 17669, and HL 06296) and by the Harry S. Moss Heart Fund. Drs Mueller and Griffin were Moss Heart Fellows and Dr Wildenthal was the recipient of a USPHS Research Career Development Award (HL 70125). The authors are grateful to J. R. Wakeland, S. C. Jasinski, P. C. Morton, and A. Vasil for expert technical assistance.
PY - 1977/7
Y1 - 1977/7
N2 - Subcutaneous injections of isoproterenol (80 mg.kg-1) in rats produce decreases in the total specific activities of several cardiac enzymes as well as redistribution of lysosomal enzymes from the particulate to the non-particulate fraction of tissue homogenates during the first day. Later, during the repair phase, activities of lysosomal enzymes increase markedly, co-incident with an influx of migratory cells, while activities of specific myocellular enzymes remain low. Methyl-prednisolone administered at the time of isoproterenol injection alters these responses, first by decreasing the extent of enzyme depletion and redistribution, and later by leading to reduced infiltration and proliferation of non-myocytic cells and reduced elevations of lysosomal enzymes during the repair phase.
AB - Subcutaneous injections of isoproterenol (80 mg.kg-1) in rats produce decreases in the total specific activities of several cardiac enzymes as well as redistribution of lysosomal enzymes from the particulate to the non-particulate fraction of tissue homogenates during the first day. Later, during the repair phase, activities of lysosomal enzymes increase markedly, co-incident with an influx of migratory cells, while activities of specific myocellular enzymes remain low. Methyl-prednisolone administered at the time of isoproterenol injection alters these responses, first by decreasing the extent of enzyme depletion and redistribution, and later by leading to reduced infiltration and proliferation of non-myocytic cells and reduced elevations of lysosomal enzymes during the repair phase.
KW - Acid phosphatase
KW - Cathepsin D, β-Acetylglucosaminidase
KW - Corticosteroids
KW - Creatine phosphokinase
KW - Lysosomal enzymes
KW - Myocardial infarction
KW - Myofibrillar alkaline proteinase
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U2 - 10.1016/S0022-2828(77)80371-4
DO - 10.1016/S0022-2828(77)80371-4
M3 - Article
C2 - 894732
AN - SCOPUS:0017408793
SN - 0022-2828
VL - 9
SP - 565
EP - 578
JO - Journal of Molecular and Cellular Cardiology
JF - Journal of Molecular and Cellular Cardiology
IS - 7
ER -