Kidney protein profiling of Wilms' tumor patients by analysis of formalin-fixed paraffin-embedded tissue samples

Elke Hammer, Florian D. Ernst, Andrea Thiele, Narasimha Kumar Karanam, Christina Kujath, Matthias Evert, Uwe Völker, Winfried Barthlen

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Wilms' tumor (nephroblastoma, WT) is the most frequent renal cancer in children. However, molecular details leading to WT have not been characterized sufficiently yet. Proteomic studies might provide new insights but are hampered by limited availability of fresh frozen tissue specimen. Therefore, we tested formalin-fixed paraffin-embedded (FFPE) tissue sections routinely collected for pathological inspection for their use in in-depth-proteomic analyses of WT samples in comparison to fresh frozen specimen. The overlap of the proteins identified was over 65%. Thus we used FFPE material from 7 patients for tandem mass spectrometry based comparison of the proteomes of WT and healthy renal tissues. We detected 262 proteins, which were differentially expressed in tumor compared to healthy renal tissue. The majority of these proteins displayed lower levels in the tumor tissue and only 30% higher levels. For selected candidates data were confirmed by immunohistochemical staining. Correlation analysis of blastemal proportions in WT and protein intensities revealed candidates for tumor stratification. Conclusion: This proof of principle proteomic study of FFPE tissue sections from WT patients demonstrates that these archived tissues constitute a valuable resource for larger in-depth proteomic studies to identify markers to follow chemotherapy efficiency or for stratification of tumor subtypes.

Original languageEnglish (US)
Pages (from-to)235-241
Number of pages7
JournalClinica Chimica Acta
Volume433
DOIs
StatePublished - Jun 10 2014
Externally publishedYes

Fingerprint

Wilms Tumor
Paraffin
Formaldehyde
Tumors
Tissue
Kidney
Proteomics
Proteins
Neoplasms
Chemotherapy
Kidney Neoplasms
Proteome
Tandem Mass Spectrometry
Mass spectrometry
Inspection
Availability
Staining and Labeling
Drug Therapy

Keywords

  • FFPE
  • Formalin-fixed paraffin-embedded material
  • Nephroblastoma
  • Proteomics
  • Wilms' tumor

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Kidney protein profiling of Wilms' tumor patients by analysis of formalin-fixed paraffin-embedded tissue samples. / Hammer, Elke; Ernst, Florian D.; Thiele, Andrea; Karanam, Narasimha Kumar; Kujath, Christina; Evert, Matthias; Völker, Uwe; Barthlen, Winfried.

In: Clinica Chimica Acta, Vol. 433, 10.06.2014, p. 235-241.

Research output: Contribution to journalArticle

Hammer, Elke ; Ernst, Florian D. ; Thiele, Andrea ; Karanam, Narasimha Kumar ; Kujath, Christina ; Evert, Matthias ; Völker, Uwe ; Barthlen, Winfried. / Kidney protein profiling of Wilms' tumor patients by analysis of formalin-fixed paraffin-embedded tissue samples. In: Clinica Chimica Acta. 2014 ; Vol. 433. pp. 235-241.
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