Label transfer chemistry for the characterization of protein-protein interactions

Bo Liu, Chase T. Archer, Lyle Burdine, Thomas G. Gillette, Thomas Kodadek

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

A new label transfer method is presented that overcomes most of the limitations of current systems. A protein of interest is tagged with a tetracysteine sequence (FlAsH receptor peptide (FRP)) that binds tightly and specifically to a chimeric molecule 3,4-dihydroxyphenylalanine-biotin-4′,5′-bis(1,3,2-dithioarsolan-2-yl)fluorescein (DOPA-biotin-FlAsH). Upon brief periodate oxidation, the DOPA moiety is cross-linked to nearby surface-exposed nucleophiles. Boiling the products in excess dithiol dissolves the FlAsH-FRP interaction, resulting in transfer of the biotin tag to the partner proteins, allowingthem to be identified by standard methods.

Original languageEnglish (US)
Pages (from-to)12348-12349
Number of pages2
JournalJournal of the American Chemical Society
Volume129
Issue number41
DOIs
StatePublished - Oct 17 2007

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Biotin
Labels
Peptide Receptors
Proteins
Peptides
Dihydroxyphenylalanine
Nucleophiles
Fluorescein
Boiling liquids
Oxidation
Molecules

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Label transfer chemistry for the characterization of protein-protein interactions. / Liu, Bo; Archer, Chase T.; Burdine, Lyle; Gillette, Thomas G.; Kodadek, Thomas.

In: Journal of the American Chemical Society, Vol. 129, No. 41, 17.10.2007, p. 12348-12349.

Research output: Contribution to journalArticle

Liu, B, Archer, CT, Burdine, L, Gillette, TG & Kodadek, T 2007, 'Label transfer chemistry for the characterization of protein-protein interactions', Journal of the American Chemical Society, vol. 129, no. 41, pp. 12348-12349. https://doi.org/10.1021/ja072904r
Liu, Bo ; Archer, Chase T. ; Burdine, Lyle ; Gillette, Thomas G. ; Kodadek, Thomas. / Label transfer chemistry for the characterization of protein-protein interactions. In: Journal of the American Chemical Society. 2007 ; Vol. 129, No. 41. pp. 12348-12349.
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