lac repressor forms loops with linear DNA carrying two suitably spaced lac operators.

H. Krämer, M. Niemöller, M. Amouyal, B. Revet, B. von Wilcken-Bergmann, B. Müller-Hill

Research output: Contribution to journalArticle

252 Citations (Scopus)

Abstract

Tetrameric lac repressor may bind to two lac operators on one DNA fragment and induce the intervening DNA to form a loop. Electron microscopy, non-denaturing polyacrylamide gel electrophoresis, and DNase I protection experiments were used to demonstrate such DNA loops, where the distance between the centres of symmetry of the two lac operators varies between 63 and 535 bp. Formation of a DNA loop is favoured by correct phasing of the two lac operators and a low concentration of both components of the reaction. When a large excess of lac repressor over DNA is used, a 'tandem' structure is observed, in which both lac operators are occupied independently by two repressor tetramers. When the concentrations of both lac repressor and lac operator are high, a 'sandwich' structure is observed, in which two DNA molecules are connected by two lac repressor tetramers in trans.

Original languageEnglish (US)
Pages (from-to)1481-1491
Number of pages11
JournalEMBO Journal
Volume6
Issue number5
StatePublished - May 1987

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Lac Repressors
DNA
Native Polyacrylamide Gel Electrophoresis
Sandwich structures
Deoxyribonuclease I
Electrophoresis
Electron microscopy
Electron Microscopy
Molecules

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Krämer, H., Niemöller, M., Amouyal, M., Revet, B., von Wilcken-Bergmann, B., & Müller-Hill, B. (1987). lac repressor forms loops with linear DNA carrying two suitably spaced lac operators. EMBO Journal, 6(5), 1481-1491.

lac repressor forms loops with linear DNA carrying two suitably spaced lac operators. / Krämer, H.; Niemöller, M.; Amouyal, M.; Revet, B.; von Wilcken-Bergmann, B.; Müller-Hill, B.

In: EMBO Journal, Vol. 6, No. 5, 05.1987, p. 1481-1491.

Research output: Contribution to journalArticle

Krämer, H, Niemöller, M, Amouyal, M, Revet, B, von Wilcken-Bergmann, B & Müller-Hill, B 1987, 'lac repressor forms loops with linear DNA carrying two suitably spaced lac operators.', EMBO Journal, vol. 6, no. 5, pp. 1481-1491.
Krämer H, Niemöller M, Amouyal M, Revet B, von Wilcken-Bergmann B, Müller-Hill B. lac repressor forms loops with linear DNA carrying two suitably spaced lac operators. EMBO Journal. 1987 May;6(5):1481-1491.
Krämer, H. ; Niemöller, M. ; Amouyal, M. ; Revet, B. ; von Wilcken-Bergmann, B. ; Müller-Hill, B. / lac repressor forms loops with linear DNA carrying two suitably spaced lac operators. In: EMBO Journal. 1987 ; Vol. 6, No. 5. pp. 1481-1491.
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