Leucine reduces reactive oxygen species levels via an energy metabolism switch by activation of the mTOR-HIF-1α pathway in porcine intestinal epithelial cells

Jun Hu, Yangfan Nie, Shifeng Chen, Chunlin Xie, Qiwen Fan, Zhichang Wang, Baisheng Long, Guokai Yan, Qing Zhong, Xianghua Yan

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Leucine serves not only as a substrate for protein synthesis, but also as a signal molecule involved in protein metabolism. However, whether the levels of cellular reactive oxygen species (ROS), which have damaging effects on cellular DNA, proteins, and lipids, are regulated by leucine is still unclear. Here, we report that leucine supplementation reduces ROS levels in intestinal epithelial cells of weaned piglets. A proteomics analysis revealed that leucine supplementation induces an energy metabolism switch from oxidative phosphorylation (OXPHOS) towards glycolysis. The leucine-induced ROS reduction and the energy metabolism switch were further validated in cultured cells. Mechanistically, our data revealed that leucine-induced ROS reduction actually depends on the energy metabolism switch from OXPHOS towards glycolysis through the mechanistic target of rapamycin (mTOR)- hypoxia-inducible factor-1alpha (HIF-1α) pathway. These findings reveal a vital regulatory role of leucine as the signal molecule involved in an energy metabolism switch in mammals.

Original languageEnglish (US)
Pages (from-to)42-56
Number of pages15
JournalInternational Journal of Biochemistry and Cell Biology
Volume89
DOIs
StatePublished - Aug 1 2017

Fingerprint

Sirolimus
Leucine
Energy Metabolism
Reactive Oxygen Species
Swine
Epithelial Cells
Chemical activation
Switches
Oxidative Phosphorylation
Glycolysis
Proteins
Molecules
Mammals
Hypoxia
Metabolism
Proteomics
Cultured Cells
Cells
Lipids
DNA

Keywords

  • Glycolysis
  • HIF-1α
  • Leucine
  • mTOR
  • Oxidative phosphorylation
  • Reactive oxygen species

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Leucine reduces reactive oxygen species levels via an energy metabolism switch by activation of the mTOR-HIF-1α pathway in porcine intestinal epithelial cells. / Hu, Jun; Nie, Yangfan; Chen, Shifeng; Xie, Chunlin; Fan, Qiwen; Wang, Zhichang; Long, Baisheng; Yan, Guokai; Zhong, Qing; Yan, Xianghua.

In: International Journal of Biochemistry and Cell Biology, Vol. 89, 01.08.2017, p. 42-56.

Research output: Contribution to journalArticle

Hu, Jun ; Nie, Yangfan ; Chen, Shifeng ; Xie, Chunlin ; Fan, Qiwen ; Wang, Zhichang ; Long, Baisheng ; Yan, Guokai ; Zhong, Qing ; Yan, Xianghua. / Leucine reduces reactive oxygen species levels via an energy metabolism switch by activation of the mTOR-HIF-1α pathway in porcine intestinal epithelial cells. In: International Journal of Biochemistry and Cell Biology. 2017 ; Vol. 89. pp. 42-56.
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AU - Fan, Qiwen

AU - Wang, Zhichang

AU - Long, Baisheng

AU - Yan, Guokai

AU - Zhong, Qing

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