Light-sheet microscopy of cleared tissues with isotropic, subcellular resolution

Tonmoy Chakraborty, Meghan K. Driscoll, Elise Jeffery, Malea M. Murphy, Philippe Roudot, Bo Jui Chang, Saumya Vora, Wen Mai Wong, Cara D. Nielson, Hua Zhang, Vladimir Zhemkov, Chitkale Hiremath, Estanislao Daniel De La Cruz, Yating Yi, Ilya Bezprozvanny, Hu Zhao, Raju Tomer, Rainer Heintzmann, Julian P. Meeks, Denise K. MarcianoSean J. Morrison, Gaudenz Danuser, Kevin M. Dean, Reto Fiolka

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We present cleared-tissue axially swept light-sheet microscopy (ctASLM), which enables isotropic, subcellular resolution imaging with high optical sectioning capability and a large field of view over a broad range of immersion media. ctASLM can image live, expanded, and both aqueous and non-aqueous chemically cleared tissue preparations. Depending on the optical configuration, ctASLM provides up to 260 nm of axial resolution, a three to tenfold improvement over confocal and other reported cleared-tissue light-sheet microscopes. We imaged millimeter-scale cleared tissues with subcellular three-dimensional resolution, which enabled automated detection of multicellular tissue architectures, individual cells, synaptic spines and rare cell–cell interactions.

Original languageEnglish (US)
Pages (from-to)1109-1113
Number of pages5
JournalNature methods
Volume16
Issue number11
DOIs
StatePublished - Nov 1 2019

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Microscopy
Microscopic examination
Tissue
Light
Immersion
Spine
Microscopes
Imaging techniques

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Chakraborty, T., Driscoll, M. K., Jeffery, E., Murphy, M. M., Roudot, P., Chang, B. J., ... Fiolka, R. (2019). Light-sheet microscopy of cleared tissues with isotropic, subcellular resolution. Nature methods, 16(11), 1109-1113. https://doi.org/10.1038/s41592-019-0615-4

Light-sheet microscopy of cleared tissues with isotropic, subcellular resolution. / Chakraborty, Tonmoy; Driscoll, Meghan K.; Jeffery, Elise; Murphy, Malea M.; Roudot, Philippe; Chang, Bo Jui; Vora, Saumya; Wong, Wen Mai; Nielson, Cara D.; Zhang, Hua; Zhemkov, Vladimir; Hiremath, Chitkale; De La Cruz, Estanislao Daniel; Yi, Yating; Bezprozvanny, Ilya; Zhao, Hu; Tomer, Raju; Heintzmann, Rainer; Meeks, Julian P.; Marciano, Denise K.; Morrison, Sean J.; Danuser, Gaudenz; Dean, Kevin M.; Fiolka, Reto.

In: Nature methods, Vol. 16, No. 11, 01.11.2019, p. 1109-1113.

Research output: Contribution to journalArticle

Chakraborty, T, Driscoll, MK, Jeffery, E, Murphy, MM, Roudot, P, Chang, BJ, Vora, S, Wong, WM, Nielson, CD, Zhang, H, Zhemkov, V, Hiremath, C, De La Cruz, ED, Yi, Y, Bezprozvanny, I, Zhao, H, Tomer, R, Heintzmann, R, Meeks, JP, Marciano, DK, Morrison, SJ, Danuser, G, Dean, KM & Fiolka, R 2019, 'Light-sheet microscopy of cleared tissues with isotropic, subcellular resolution', Nature methods, vol. 16, no. 11, pp. 1109-1113. https://doi.org/10.1038/s41592-019-0615-4
Chakraborty, Tonmoy ; Driscoll, Meghan K. ; Jeffery, Elise ; Murphy, Malea M. ; Roudot, Philippe ; Chang, Bo Jui ; Vora, Saumya ; Wong, Wen Mai ; Nielson, Cara D. ; Zhang, Hua ; Zhemkov, Vladimir ; Hiremath, Chitkale ; De La Cruz, Estanislao Daniel ; Yi, Yating ; Bezprozvanny, Ilya ; Zhao, Hu ; Tomer, Raju ; Heintzmann, Rainer ; Meeks, Julian P. ; Marciano, Denise K. ; Morrison, Sean J. ; Danuser, Gaudenz ; Dean, Kevin M. ; Fiolka, Reto. / Light-sheet microscopy of cleared tissues with isotropic, subcellular resolution. In: Nature methods. 2019 ; Vol. 16, No. 11. pp. 1109-1113.
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AU - Chang, Bo Jui

AU - Vora, Saumya

AU - Wong, Wen Mai

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AU - Tomer, Raju

AU - Heintzmann, Rainer

AU - Meeks, Julian P.

AU - Marciano, Denise K.

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