@article{adfb91c48714471181c185180e4b61d1,
title = "Light-sheet microscopy of cleared tissues with isotropic, subcellular resolution",
abstract = "We present cleared-tissue axially swept light-sheet microscopy (ctASLM), which enables isotropic, subcellular resolution imaging with high optical sectioning capability and a large field of view over a broad range of immersion media. ctASLM can image live, expanded, and both aqueous and non-aqueous chemically cleared tissue preparations. Depending on the optical configuration, ctASLM provides up to 260 nm of axial resolution, a three to tenfold improvement over confocal and other reported cleared-tissue light-sheet microscopes. We imaged millimeter-scale cleared tissues with subcellular three-dimensional resolution, which enabled automated detection of multicellular tissue architectures, individual cells, synaptic spines and rare cell–cell interactions.",
author = "Tonmoy Chakraborty and Driscoll, {Meghan K.} and Elise Jeffery and Murphy, {Malea M.} and Philippe Roudot and Chang, {Bo Jui} and Saumya Vora and Wong, {Wen Mai} and Nielson, {Cara D.} and Hua Zhang and Vladimir Zhemkov and Chitkale Hiremath and {De La Cruz}, {Estanislao Daniel} and Yating Yi and Ilya Bezprozvanny and Hu Zhao and Raju Tomer and Rainer Heintzmann and Meeks, {Julian P.} and Marciano, {Denise K.} and Morrison, {Sean J.} and Gaudenz Danuser and Dean, {Kevin M.} and Reto Fiolka",
note = "Funding Information: We thank the Cancer Prevention Research Institute of Texas (grant nos. RR160057 to R.F. and R1225 to G.D.) for their generous funding, as well as the National Institutes of Health (grant nos. F32GM116370 and K99GM123221 to M.K.D., R01GM067230 to G.D., R01AG055577 and R01NS056224 to I.B., R01DK118032 and R01DK099478 to D.M., R01DC015784 and R21NS104826 to J.M., R33CA235254 and R35GM133522 to R.F., and DP2MH119423 to R.T.). R.H. acknowledges support by the Collaborative Research Center SFB 1278 (PolyTarget, project C04) funded by the Deutsche Forschungsgemeinschaft. We are grateful to the Live cell imaging facility at UT Southwestern for access to the Zeiss Airyscan confocal microscope (supported by grant no. 1S10OD021684-01). We thank D. Saucier and J. Amatruda for providing the zebrafish specimens and E. Sapoznik for his assistance with computer-aided design. Publisher Copyright: {\textcopyright} 2019, The Author(s), under exclusive licence to Springer Nature America, Inc.",
year = "2019",
month = nov,
day = "1",
doi = "10.1038/s41592-019-0615-4",
language = "English (US)",
volume = "16",
pages = "1109--1113",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Public Library of Science",
number = "11",
}