Light-sheet microscopy of cleared tissues with isotropic, subcellular resolution

Tonmoy Chakraborty, Meghan K. Driscoll, Elise Jeffery, Malea M. Murphy, Philippe Roudot, Bo Jui Chang, Saumya Vora, Wen Mai Wong, Cara D. Nielson, Hua Zhang, Vladimir Zhemkov, Chitkale Hiremath, Estanislao Daniel De La Cruz, Yating Yi, Ilya Bezprozvanny, Hu Zhao, Raju Tomer, Rainer Heintzmann, Julian P. Meeks, Denise K. MarcianoSean J. Morrison, Gaudenz Danuser, Kevin M. Dean, Reto Fiolka

Research output: Contribution to journal › Article

1 Citation (Scopus)

Abstract

We present cleared-tissue axially swept light-sheet microscopy (ctASLM), which enables isotropic, subcellular resolution imaging with high optical sectioning capability and a large field of view over a broad range of immersion media. ctASLM can image live, expanded, and both aqueous and non-aqueous chemically cleared tissue preparations. Depending on the optical configuration, ctASLM provides up to 260 nm of axial resolution, a three to tenfold improvement over confocal and other reported cleared-tissue light-sheet microscopes. We imaged millimeter-scale cleared tissues with subcellular three-dimensional resolution, which enabled automated detection of multicellular tissue architectures, individual cells, synaptic spines and rare cell–cell interactions.

Original languageEnglish (US)
Pages (from-to)1109-1113
Number of pages5
JournalNature methods
Volume16
Issue number11
DOIs
StatePublished - Nov 1 2019

Fingerprint

Microscopy
Microscopic examination
Tissue
Light
Immersion
Spine
Microscopes
Imaging techniques

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Chakraborty, T., Driscoll, M. K., Jeffery, E., Murphy, M. M., Roudot, P., Chang, B. J., ... Fiolka, R. (2019). Light-sheet microscopy of cleared tissues with isotropic, subcellular resolution. Nature methods, 16(11), 1109-1113. https://doi.org/10.1038/s41592-019-0615-4

Light-sheet microscopy of cleared tissues with isotropic, subcellular resolution. / Chakraborty, Tonmoy; Driscoll, Meghan K.; Jeffery, Elise; Murphy, Malea M.; Roudot, Philippe; Chang, Bo Jui; Vora, Saumya; Wong, Wen Mai; Nielson, Cara D.; Zhang, Hua; Zhemkov, Vladimir; Hiremath, Chitkale; De La Cruz, Estanislao Daniel; Yi, Yating; Bezprozvanny, Ilya; Zhao, Hu; Tomer, Raju; Heintzmann, Rainer; Meeks, Julian P.; Marciano, Denise K.; Morrison, Sean J.; Danuser, Gaudenz; Dean, Kevin M.; Fiolka, Reto.

In: Nature methods, Vol. 16, No. 11, 01.11.2019, p. 1109-1113.

Research output: Contribution to journal › Article

Chakraborty, T, Driscoll, MK, Jeffery, E, Murphy, MM, Roudot, P, Chang, BJ, Vora, S, Wong, WM, Nielson, CD, Zhang, H, Zhemkov, V, Hiremath, C, De La Cruz, ED, Yi, Y, Bezprozvanny, I, Zhao, H, Tomer, R, Heintzmann, R, Meeks, JP, Marciano, DK, Morrison, SJ, Danuser, G, Dean, KM & Fiolka, R 2019, 'Light-sheet microscopy of cleared tissues with isotropic, subcellular resolution', Nature methods, vol. 16, no. 11, pp. 1109-1113. https://doi.org/10.1038/s41592-019-0615-4
Chakraborty T, Driscoll MK, Jeffery E, Murphy MM, Roudot P, Chang BJ et al. Light-sheet microscopy of cleared tissues with isotropic, subcellular resolution. Nature methods. 2019 Nov 1;16(11):1109-1113. https://doi.org/10.1038/s41592-019-0615-4
Chakraborty, Tonmoy ; Driscoll, Meghan K. ; Jeffery, Elise ; Murphy, Malea M. ; Roudot, Philippe ; Chang, Bo Jui ; Vora, Saumya ; Wong, Wen Mai ; Nielson, Cara D. ; Zhang, Hua ; Zhemkov, Vladimir ; Hiremath, Chitkale ; De La Cruz, Estanislao Daniel ; Yi, Yating ; Bezprozvanny, Ilya ; Zhao, Hu ; Tomer, Raju ; Heintzmann, Rainer ; Meeks, Julian P. ; Marciano, Denise K. ; Morrison, Sean J. ; Danuser, Gaudenz ; Dean, Kevin M. ; Fiolka, Reto. / Light-sheet microscopy of cleared tissues with isotropic, subcellular resolution. In: Nature methods. 2019 ; Vol. 16, No. 11. pp. 1109-1113.
@article{adfb91c48714471181c185180e4b61d1,
title = "Light-sheet microscopy of cleared tissues with isotropic, subcellular resolution",
abstract = "We present cleared-tissue axially swept light-sheet microscopy (ctASLM), which enables isotropic, subcellular resolution imaging with high optical sectioning capability and a large field of view over a broad range of immersion media. ctASLM can image live, expanded, and both aqueous and non-aqueous chemically cleared tissue preparations. Depending on the optical configuration, ctASLM provides up to 260 nm of axial resolution, a three to tenfold improvement over confocal and other reported cleared-tissue light-sheet microscopes. We imaged millimeter-scale cleared tissues with subcellular three-dimensional resolution, which enabled automated detection of multicellular tissue architectures, individual cells, synaptic spines and rare cell–cell interactions.",
author = "Tonmoy Chakraborty and Driscoll, {Meghan K.} and Elise Jeffery and Murphy, {Malea M.} and Philippe Roudot and Chang, {Bo Jui} and Saumya Vora and Wong, {Wen Mai} and Nielson, {Cara D.} and Hua Zhang and Vladimir Zhemkov and Chitkale Hiremath and {De La Cruz}, {Estanislao Daniel} and Yating Yi and Ilya Bezprozvanny and Hu Zhao and Raju Tomer and Rainer Heintzmann and Meeks, {Julian P.} and Marciano, {Denise K.} and Morrison, {Sean J.} and Gaudenz Danuser and Dean, {Kevin M.} and Reto Fiolka",
year = "2019",
month = "11",
day = "1",
doi = "10.1038/s41592-019-0615-4",
language = "English (US)",
volume = "16",
pages = "1109--1113",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Public Library of Science",
number = "11",

}

TY - JOUR

T1 - Light-sheet microscopy of cleared tissues with isotropic, subcellular resolution

AU - Chakraborty, Tonmoy

AU - Driscoll, Meghan K.

AU - Jeffery, Elise

AU - Murphy, Malea M.

AU - Roudot, Philippe

AU - Chang, Bo Jui

AU - Vora, Saumya

AU - Wong, Wen Mai

AU - Nielson, Cara D.

AU - Zhang, Hua

AU - Zhemkov, Vladimir

AU - Hiremath, Chitkale

AU - De La Cruz, Estanislao Daniel

AU - Yi, Yating

AU - Bezprozvanny, Ilya

AU - Zhao, Hu

AU - Tomer, Raju

AU - Heintzmann, Rainer

AU - Meeks, Julian P.

AU - Marciano, Denise K.

AU - Morrison, Sean J.

AU - Danuser, Gaudenz

AU - Dean, Kevin M.

AU - Fiolka, Reto

PY - 2019/11/1

Y1 - 2019/11/1

N2 - We present cleared-tissue axially swept light-sheet microscopy (ctASLM), which enables isotropic, subcellular resolution imaging with high optical sectioning capability and a large field of view over a broad range of immersion media. ctASLM can image live, expanded, and both aqueous and non-aqueous chemically cleared tissue preparations. Depending on the optical configuration, ctASLM provides up to 260 nm of axial resolution, a three to tenfold improvement over confocal and other reported cleared-tissue light-sheet microscopes. We imaged millimeter-scale cleared tissues with subcellular three-dimensional resolution, which enabled automated detection of multicellular tissue architectures, individual cells, synaptic spines and rare cell–cell interactions.

AB - We present cleared-tissue axially swept light-sheet microscopy (ctASLM), which enables isotropic, subcellular resolution imaging with high optical sectioning capability and a large field of view over a broad range of immersion media. ctASLM can image live, expanded, and both aqueous and non-aqueous chemically cleared tissue preparations. Depending on the optical configuration, ctASLM provides up to 260 nm of axial resolution, a three to tenfold improvement over confocal and other reported cleared-tissue light-sheet microscopes. We imaged millimeter-scale cleared tissues with subcellular three-dimensional resolution, which enabled automated detection of multicellular tissue architectures, individual cells, synaptic spines and rare cell–cell interactions.

UR - http://www.scopus.com/inward/record.url?scp=85074283217&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85074283217&partnerID=8YFLogxK

U2 - 10.1038/s41592-019-0615-4

DO - 10.1038/s41592-019-0615-4

M3 - Article

C2 - 31673159

AN - SCOPUS:85074283217

VL - 16

SP - 1109

EP - 1113

JO - Nature Methods

JF - Nature Methods

SN - 1548-7091

IS - 11

ER -

Access to Document