Linking spermatid ribonucleic acid (RNA) binding protein and retrogene diversity to reproductive success

Karen M. Chapman, Heather M. Powell, Jaideep Chaudhary, John M. Shelton, James A. Richardson, Timothy E. Richardson, F. Kent Hamra

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Spermiogenesis is a postmeiotic process that drives development of round spermatids into fully elongated spermatozoa. Spermatid elongation is largely controlled posttranscriptionally after global silencing of mRNA synthesis from the haploid genome. Here, rats that differentially express EGFP from a lentiviral transgene during early and late steps of spermiogenesis were used to flow sort fractions of round and elongating spermatids. Mass-spectral analysis of 2D gel protein spots enriched >3-fold in each fraction revealed a heterogeneous RNA binding proteome (hnRNPA2/b1, hnRNPA3, hnRPDL, hnRNPK, hnRNPL, hnRNPM, PABPC1, PABPC4, PCBP1, PCBP3, PTBP2, PSIP1, RGSL1, RUVBL2, SARNP2, TDRD6, TDRD7) abundantly expressed in round spermatids prior to their elongation. Notably, each protein within this ontology cluster regulates alternative splicing, sub-cellular transport, degradation and/or translational repression of mRNAs. In contrast, elongating spermatid fractions were enriched with glycolytic enzymes, redox enzymes and protein synthesis factors. Retrogene-encoded proteins were over-represented among the most abundant elongating spermatid factors identified. Consistent with these biochemical activities, plus corresponding histological profiles, the identified RNA processing factors are predicted to collectively drive post-transcriptional expression of an alternative exome that fuels finishing steps of sperm maturation and fitness.

Original languageEnglish (US)
Pages (from-to)3221-3236
Number of pages16
JournalMolecular and Cellular Proteomics
Volume12
Issue number11
DOIs
StatePublished - Nov 2013

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Spermatids
Carrier Proteins
RNA
Elongation
Proteins
Spermatogenesis
Messenger RNA
Alternative Splicing
Enzymes
Proteome
Sperm Maturation
Spectrum analysis
Exome
Ontology
Rats
Genes
Gels
Haploidy
Transgenes
Degradation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Analytical Chemistry

Cite this

Linking spermatid ribonucleic acid (RNA) binding protein and retrogene diversity to reproductive success. / Chapman, Karen M.; Powell, Heather M.; Chaudhary, Jaideep; Shelton, John M.; Richardson, James A.; Richardson, Timothy E.; Hamra, F. Kent.

In: Molecular and Cellular Proteomics, Vol. 12, No. 11, 11.2013, p. 3221-3236.

Research output: Contribution to journalArticle

Chapman, Karen M. ; Powell, Heather M. ; Chaudhary, Jaideep ; Shelton, John M. ; Richardson, James A. ; Richardson, Timothy E. ; Hamra, F. Kent. / Linking spermatid ribonucleic acid (RNA) binding protein and retrogene diversity to reproductive success. In: Molecular and Cellular Proteomics. 2013 ; Vol. 12, No. 11. pp. 3221-3236.
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