Localization of mRNA for low density lipoprotein receptor and a cholesterol synthetic enzyme in rabbit nervous system by in situ hybridization

L. W. Swanson, D. M. Simmons, S. L. Hofmann, J. L. Goldstein, M. S. Brown

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Abstract

The low density lipoprotein receptor and one of its ligands, apoprotein E, are known to be synthesized in the central nervous system. In the current study, we used in situ hybridization to localize the receptor mRNA in selected neurons and glia throughout the nervous system of 9-day-old rabbits. Particularly high levels were found in sensory ganglia, sensory nuclei, and motor-related nuclei. The same regions contained high levels of mRNA for 3-hydroxy-3-methylglutaryl-coenzyme A synthase, a regulated enzyme in cholesterol biosynthesis. The distribution of low density lipoprotein receptor mRNA was similar in mature and immature rabbits. The data suggest that certain cells in the nervous system have high requirements for cholesterol, which they satisfy through cholesterol synthesis and through receptor-mediated uptake of cholesterol-carrying lipoproteins. The latter originate in astrocytes which synthesize and secrete apoprotein E. These data suggest that the nervous system of mammals contains an active system for continuous redistribution and recycling of cholesterol that is physically distinct from the lipoprotein transport system in plasma.

Original languageEnglish (US)
Pages (from-to)9821-9825
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number24
StatePublished - 1988

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Lipoprotein(a)
LDL Receptors
Nervous System
In Situ Hybridization
Cholesterol
Rabbits
Messenger RNA
Apolipoproteins E
Enzymes
Hydroxymethylglutaryl-CoA Synthase
Sensory Ganglia
Recycling
Neuroglia
Astrocytes
Lipoproteins
Mammals
Central Nervous System
Ligands
Neurons

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

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title = "Localization of mRNA for low density lipoprotein receptor and a cholesterol synthetic enzyme in rabbit nervous system by in situ hybridization",
abstract = "The low density lipoprotein receptor and one of its ligands, apoprotein E, are known to be synthesized in the central nervous system. In the current study, we used in situ hybridization to localize the receptor mRNA in selected neurons and glia throughout the nervous system of 9-day-old rabbits. Particularly high levels were found in sensory ganglia, sensory nuclei, and motor-related nuclei. The same regions contained high levels of mRNA for 3-hydroxy-3-methylglutaryl-coenzyme A synthase, a regulated enzyme in cholesterol biosynthesis. The distribution of low density lipoprotein receptor mRNA was similar in mature and immature rabbits. The data suggest that certain cells in the nervous system have high requirements for cholesterol, which they satisfy through cholesterol synthesis and through receptor-mediated uptake of cholesterol-carrying lipoproteins. The latter originate in astrocytes which synthesize and secrete apoprotein E. These data suggest that the nervous system of mammals contains an active system for continuous redistribution and recycling of cholesterol that is physically distinct from the lipoprotein transport system in plasma.",
author = "Swanson, {L. W.} and Simmons, {D. M.} and Hofmann, {S. L.} and Goldstein, {J. L.} and Brown, {M. S.}",
year = "1988",
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T1 - Localization of mRNA for low density lipoprotein receptor and a cholesterol synthetic enzyme in rabbit nervous system by in situ hybridization

AU - Swanson, L. W.

AU - Simmons, D. M.

AU - Hofmann, S. L.

AU - Goldstein, J. L.

AU - Brown, M. S.

PY - 1988

Y1 - 1988

N2 - The low density lipoprotein receptor and one of its ligands, apoprotein E, are known to be synthesized in the central nervous system. In the current study, we used in situ hybridization to localize the receptor mRNA in selected neurons and glia throughout the nervous system of 9-day-old rabbits. Particularly high levels were found in sensory ganglia, sensory nuclei, and motor-related nuclei. The same regions contained high levels of mRNA for 3-hydroxy-3-methylglutaryl-coenzyme A synthase, a regulated enzyme in cholesterol biosynthesis. The distribution of low density lipoprotein receptor mRNA was similar in mature and immature rabbits. The data suggest that certain cells in the nervous system have high requirements for cholesterol, which they satisfy through cholesterol synthesis and through receptor-mediated uptake of cholesterol-carrying lipoproteins. The latter originate in astrocytes which synthesize and secrete apoprotein E. These data suggest that the nervous system of mammals contains an active system for continuous redistribution and recycling of cholesterol that is physically distinct from the lipoprotein transport system in plasma.

AB - The low density lipoprotein receptor and one of its ligands, apoprotein E, are known to be synthesized in the central nervous system. In the current study, we used in situ hybridization to localize the receptor mRNA in selected neurons and glia throughout the nervous system of 9-day-old rabbits. Particularly high levels were found in sensory ganglia, sensory nuclei, and motor-related nuclei. The same regions contained high levels of mRNA for 3-hydroxy-3-methylglutaryl-coenzyme A synthase, a regulated enzyme in cholesterol biosynthesis. The distribution of low density lipoprotein receptor mRNA was similar in mature and immature rabbits. The data suggest that certain cells in the nervous system have high requirements for cholesterol, which they satisfy through cholesterol synthesis and through receptor-mediated uptake of cholesterol-carrying lipoproteins. The latter originate in astrocytes which synthesize and secrete apoprotein E. These data suggest that the nervous system of mammals contains an active system for continuous redistribution and recycling of cholesterol that is physically distinct from the lipoprotein transport system in plasma.

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