The numerous biological roles of NAD+ are organized and coordinated via its compartmentalization within cells. The spatial and temporal partitioning of this intermediary metabolite is intrinsic to understanding the impact of NAD+ on cellular signaling and metabolism. We review evidence supporting the compartmentalization of steady-state NAD+ levels in cells, as well as how the modulation of NAD+ synthesis dynamically regulates signaling by controlling subcellular NAD+ concentrations. We further discuss potential benefits to the cell of compartmentalizing NAD+, and methods for measuring subcellular NAD+ levels.
- nicotinamide mononucleotide adenylyltransferase (NMNAT)
- nicotinamide phosphoribosyltransferase (NAMPT)
- poly(ADP-ribose) polymerase (PARP)
ASJC Scopus subject areas
- Molecular Biology