TY - JOUR
T1 - Location, Location, Location
T2 - Compartmentalization of NAD+ Synthesis and Functions in Mammalian Cells
AU - Cambronne, Xiaolu A.
AU - Kraus, W. Lee
N1 - Funding Information:
We thank A. Jones, K. Ryu, S. Challa, J. Eller, and M. Stokes for critical comments and suggestions on this work. NAD+-related research in our laboratories is supported by the National Institutes of Health (NIH)/National Institute of General Medical Sciences (NIGMS) grant DP2GM126897 (to X.A.C.), and by the NIH National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) grant R01 DK069710 and funds from the Cecil H. and Ida Green Center for Reproductive Biology Sciences Endowment (to W.L.K.). X.A.C. is an inventor on US patent 10 392 649 covering the NAD+ cpVenus-based sensor described herein. W.L.K. is a founder and consultant for Ribon Therapeutics. He is also an inventor on US patent 9 599 606 covering a set of ADP-ribose detection reagents which have been licensed to and are sold by EMD Millipore.
Funding Information:
We thank A. Jones, K. Ryu, S. Challa, J. Eller, and M. Stokes for critical comments and suggestions on this work. NAD + -related research in our laboratories is supported by the National Institutes of Health (NIH)/ National Institute of General Medical Sciences (NIGMS) grant DP2GM126897 (to X.A.C.), and by the NIH National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) grant R01 DK069710 and funds from the Cecil H. and Ida Green Center for Reproductive Biology Sciences Endowment (to W.L.K.).
Publisher Copyright:
© 2020 Elsevier Ltd
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/10
Y1 - 2020/10
N2 - The numerous biological roles of NAD+ are organized and coordinated via its compartmentalization within cells. The spatial and temporal partitioning of this intermediary metabolite is intrinsic to understanding the impact of NAD+ on cellular signaling and metabolism. We review evidence supporting the compartmentalization of steady-state NAD+ levels in cells, as well as how the modulation of NAD+ synthesis dynamically regulates signaling by controlling subcellular NAD+ concentrations. We further discuss potential benefits to the cell of compartmentalizing NAD+, and methods for measuring subcellular NAD+ levels.
AB - The numerous biological roles of NAD+ are organized and coordinated via its compartmentalization within cells. The spatial and temporal partitioning of this intermediary metabolite is intrinsic to understanding the impact of NAD+ on cellular signaling and metabolism. We review evidence supporting the compartmentalization of steady-state NAD+ levels in cells, as well as how the modulation of NAD+ synthesis dynamically regulates signaling by controlling subcellular NAD+ concentrations. We further discuss potential benefits to the cell of compartmentalizing NAD+, and methods for measuring subcellular NAD+ levels.
KW - CD38
KW - nicotinamide mononucleotide adenylyltransferase (NMNAT)
KW - nicotinamide phosphoribosyltransferase (NAMPT)
KW - poly(ADP-ribose) polymerase (PARP)
KW - sirtuin
UR - http://www.scopus.com/inward/record.url?scp=85086945662&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85086945662&partnerID=8YFLogxK
U2 - 10.1016/j.tibs.2020.05.010
DO - 10.1016/j.tibs.2020.05.010
M3 - Review article
C2 - 32595066
AN - SCOPUS:85086945662
VL - 45
SP - 858
EP - 873
JO - Trends in Biochemical Sciences
JF - Trends in Biochemical Sciences
SN - 0376-5067
IS - 10
ER -