Long- and short-range signals control the dynamic expression of an animal hemisphere-specific gene in Xenopus

Adnan Mir, Matthew Kofron, Janet Heasman, Melissa Mogle, Stephanie Lang, Bilge Birsoy, Chris Wylie

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Little is known of the control of gene expression in the animal hemisphere of the Xenopus embryo. Here we show that expression of FoxI1e, a gene essential for normal ectoderm formation, is expressed regionally within the animal hemisphere, in a highly dynamic fashion. In situ hybridization shows that FoxI1e is expressed in a wave-like fashion that is initiated on the dorsal side of the animal hemisphere, extends across to the ventral side by the mid-gastrula stage, and is then turned off in the dorsal ectoderm, the neural plate, at the neurula stage. It is confined to the inner layers of cells in the animal cap, and is expressed in a mosaic fashion throughout. We show that this dynamic pattern of expression is controlled by both short- and long-range signals. Notch signaling controls both the mosaic, and dorsal/ventral changes in expression, and is controlled, in turn, by Vg1 signaling from the vegetal mass. FoxI1e expression is also regulated by nodal signaling downstream of VegT. Canonical Wnt signaling contributes only to late changes in the FoxI1e expression pattern. These results provide new insights into the roles of vegetally localized mRNAs in controlling zygotic genes expressed in the animal hemisphere by long-range signaling. They also provide novel insights into the role of Notch signaling at the earliest stages of vertebrate development.

Original languageEnglish (US)
Pages (from-to)161-172
Number of pages12
JournalDevelopmental Biology
Volume315
Issue number1
DOIs
StatePublished - Mar 1 2008
Externally publishedYes

Fingerprint

Xenopus
Ectoderm
Genes
Neural Plate
Gastrula
Essential Genes
In Situ Hybridization
Vertebrates
Embryonic Structures
Gene Expression
Messenger RNA

Keywords

  • Animal gene expression
  • Ectoderm
  • FoxI1e
  • Xenopus

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Long- and short-range signals control the dynamic expression of an animal hemisphere-specific gene in Xenopus. / Mir, Adnan; Kofron, Matthew; Heasman, Janet; Mogle, Melissa; Lang, Stephanie; Birsoy, Bilge; Wylie, Chris.

In: Developmental Biology, Vol. 315, No. 1, 01.03.2008, p. 161-172.

Research output: Contribution to journalArticle

Mir, Adnan ; Kofron, Matthew ; Heasman, Janet ; Mogle, Melissa ; Lang, Stephanie ; Birsoy, Bilge ; Wylie, Chris. / Long- and short-range signals control the dynamic expression of an animal hemisphere-specific gene in Xenopus. In: Developmental Biology. 2008 ; Vol. 315, No. 1. pp. 161-172.
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