Abstract
A novel receptor for oxidized low-density lipoprotein (OxLDL), lectin-like OxLDL receptor (LOX-1), was cloned from endothelial cells. Since OxLDL is taken up by vascular smooth muscle cells (VSMC) in atheroma, we analyzed the inducible expression of LOX-1 in VSMC in the present study. Incubation of cultured bovine VSMC with lysophosphatidylcholine (LPC), an atherogenic component of OxLDL, increased the level of mRNA for LOX-1 in a dose- and time-dependent manner. Since LPC did not significantly change the half-life of LOX-1 mRNA, the induction seemed to occur at the transcriptional level. The induction accompanied an increase in the protein level of LOX-1 and activity of OxLDL uptake. Blocking antibody against LOX-1 significantly suppressed the enhanced uptake of OxLDL. Thus, LOX-1 is a major receptor for OxLDL in VSMC as in endothelial cells. The enhanced expression of LOX-1 by LPC suggests that OxLDL and LPC would progressively change the function of VSMC and accelerate atherogenesis in vivo. Copyright (C) 2000 Federation of European Biochemical Societies.
Original language | English (US) |
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Pages (from-to) | 217-220 |
Number of pages | 4 |
Journal | FEBS Letters |
Volume | 467 |
Issue number | 2-3 |
DOIs | |
State | Published - Feb 11 2000 |
Keywords
- Atherosclerosis
- Lectin-like oxidized low-density lipoprotein receptor
- Lysophosphatidylcholine
- Oxidized LDL
- Vascular smooth muscle cell
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology